【作者】 薛鲁燕； 黄锡荣；

【Author】 Luyan Xue,Xirong Huang~* Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University,Jinan 250100

【机构】 山东大学胶体与界面化学教育部重点实验室；

【摘要】 用疏水性离子液体取代挥发性有机溶剂配制适宜于酶活力表达的离子液体基微乳液是一个很有意义的尝试。我们观察到,借助非离子表面活性剂Triton X-100,AOT可以与疏水性离子液体[Bmim][PF₆]混溶并形成较大区域的单相微乳液。通过探针K₃Fe（CN）₆在单相微乳液相区中扩散系数随含水量的显著变化,区分出了water-in-[Bmim][P₆]（W/IL）微乳液相区。紫外可见光谱证实了该微乳液相区可以增溶无机盐CoCl₂。结合W/IL微乳液各组分的红外光谱特征,我们用重水取代普通水,首次用FTIR证明了W/IL微乳液中本体水的存在。研究还观察到,通过调节微乳液界面组成,可以提高增溶在该微乳液中漆酶的催化活力。更多还原

【Abstract】 It would be an interesting attempt to construct an ionic liquid-based reverse microemulsion suitable for the expression of the catalytic activity of an enzyme.With the aid of nonionic surfactant Triton X-100,AOT could be dissolved in hydrophobic ionic liquid[Bmim][PF6],forming a large single phase microemulsion region.The water-in-[Bmim][PF₆]（W/IL） microemulsion domain was identified electrochemically by using K₃Fe（CN）₆ as a probe.The existence of W/IL microemulsions was demonstrated spectrophotometrically by using CoCl₂ as a probe.New evidences from the FTIR spectroscopic study, which was first introduced to the W/IL microemulsion by substituting D₂O for H₂O to eliminate the spectral interference,demonstrated that there existed bulk water at largerω₀ values（coo was defined as the molar ratio of water to the total surfactant） in the W/IL microemulsion.The laccase hosted in the microemulsion exhibited a catalytic activity and the activity could be regulated by the composition of the interfacial membrane.更多还原