【作者】 张晓清； 马莉； 丁敏；

【Author】 ZHANG Xiao-qing,MA Li and DING Min~*(Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education, Chongqing Medical University,Chongqing 400016)

【机构】 重庆医科大学医学检验系临床检验诊断学教育部重点实验室；

【摘要】 本研究利用高效液相色谱紫外检测建立了一种快速、简便的测定血浆犬尿氨酸和色氨酸浓度的方法,基于犬尿氨酸和色氨酸浓度的比值与IDO酶活性的关系来间接评估IDO酶活性,并将该方法用于测定川崎病、过敏性紫癜、肾病综合征、慢性肾炎和肺炎患儿血浆色氨酸和犬尿氨酸含量,评估各疾病组IDO酶活性。结果显示,慢性肾炎和肺炎组犬尿氨酸和色氨酸浓度的比值无显著性变化,即IDO酶活性无显著变化,而川崎病组色氨酸浓度降低伴随犬尿氨酸浓度升高,提示IDO酶活性显著升高;过敏性紫癜组犬尿氨酸浓度降低,色氨酸浓度无显著性变化,二者比值显著降低,提示IDO酶活性显著降低。更多还原

【Abstract】 A simple and fast determination of kynurenine and tryptophan in plasma was established by HPLC-UV.The activity of indoleamine 2,3-dioxygenase(IDO) was estimated indirectly by the ratio of kynurenine and tryptophan. And this method was used to determine kynurenine and tryptophan in plasma of children patients with Kawasaki disease (KD),allergic purpura(AP),nephritic syndrome(NS),chronic nephritis and pneumonia and then the activity of IDO was estimated in each group.The result showed that the ratio of kynurenine and tryptophan didn’ t change significantly and the activity of IDO didn’t change obviously in chronic nephritis and pneunonia,however,the concentration of tryptophan decreased while the concentration of kynurenine increased,indicating the activity of IDO increased significantly in KD and the concentration of Kyn decreased significantly while the concentration of Trp changed little and the ratio of Kyn and Trp decreased significantly,indicating the activity of IDO decreased significantly in AP.更多还原