【作者】 苏邵； 宋世平； 樊春海；

【Author】 SU Shao,SONG Shi-ping~* and FAN Chun-hai~*(Shanghai Institute of Applied Physics,Chinese Academy of Sciences, Shanghai 201800)

【机构】 中科院上海应用物理研究所；

【摘要】 制备了一种基于硅纳米材料的IL-8的电化学传感器。经典的酶联免疫检测方法利用抗原-抗体反应的特异性并结合酶联信号放大进行检测。我们通过将捕获探针抗体组装到硅线修饰的玻碳电极上,经抗原反应后再加入信号探针抗体及酶标二抗,通过检测HRP催化底物所产生的氧化还原电流变化来检测IL-8。该传感器在1～10 ng/mL的检测范围内有很好的响应,检测限为100 pg/mL。更多还原

【Abstract】 Herein we reported an electrochemical biosensor for IL-8 detection based on silicon nanowires.Classic ELISA was based on the immunoreaction between antigen and antibody with the enzyme amplification.In this work, the capture probe antibody was immobilized on the silicon nanowires modified glass carbon electrode.After the antigen reacted,the signal probe antibody and the enzyme labeled secondary antibody were added.We then electrochemically interrogated the electrical current for peroxidase-catalyzed hydrogen peroxide.The electrochemical responses were linear with IL-8 concentrations in the range from 1 to 10 ng/mL,the detect limit was 100 pg/mL.更多还原