【作者】 施晓婷； 张君； 王林平； 图布新； 郭伟； 袁倬斌；

【Author】 SHI Xiao-ting~1,ZHANG Jun~(*1),WANG Lin-ping~1,Tubshin Hreid~1,GUO Wei~2 and YUAN Zhuo-bin~3(1.College of Chemistry and Chemical Engineering,Inner Mongolia University Hohhot 010021;2.College of Environmental and Resource Sciences of Inner Mongolia University,Hohhot 010021;3.College of Chemistry and Chemical Engineering, Graduate University of the Chinese Academy of Sciences,Beijing 100049)

【机构】 内蒙古大学化学化工学院； 内蒙古大学环境与资源学院； 中国科学院研究生院化学与化学工程学院；

【摘要】 原子力显微镜(AFM)具有分辨率高、样品制备简单以及可在保持样品生物活性条件下成像等优点。本文用原子力显微术研究了牛血清白蛋白(BSA)在六亚甲基四胺-盐酸缓冲溶液中的形态变化。牛血清白蛋白的AFM图像表明:BSA在去离子水中呈相互分散的椭圆形貌,在0.05 mol/L的六亚甲基四胺-盐酸缓冲溶液中BSA发生展开并相互粘连,其三级结构发生了明显改变。更多还原

【Abstract】 Atomic force microscopy(AFM) with a high resolution,easy sample preparation,as well as in maintaining the biological activity under the conditions of imaging,etc.,In this paper,we use atomic force microscopy(AFM) study the morphological changes of bovine serum albumin(BSA) in the six-methylene-4-amine-hydrochloric acid buffer solution,the AFM images of bovine serum albumin(BSA) show that:BSA in de-ionized water is elliptic protein.The structure of BSA changed dramatically in the 0.05 mol/L of hexamethylene tetramine-hydrochloric acid buffer solution.更多还原