【作者】 庄惠生； 王丽丽； 周纯；

【Author】 ZHUANG Hui-sheng~(1,2),WANG Li-li~2 and ZHOU Chun~2(1.School of Environmental Science and Engineering, Shanghai Jiaotong University,800 Dongchuan Road,Minhang,Shanghai 200240;2.College of Environmental Sci- ence and Engineering,Donghua University,Shanghai 201620)

【机构】 上海交通大学环境科学与工程学院； 东华大学环境科学与工程学院；

【摘要】 使用自制的LC标记到NA和PH的人工抗体上,建立了直接竞争荧光免疫分析法测定NA和PH,测定NA标准曲线的线性范围:1.0×10^-7～1.0×10^-2mg/mL,线性方程:y=7.5053x+61.536,R²=0.9917,最低检测限（LOD）为5.0×10^-8mg/mL,测定生活污泥,印染污泥和电镀污泥等三种污泥中的NA,回收率为97.53%～107.57%。测定PH标准曲线的线性范围:1.0×10^-6～2.0×10^-4mg/L,线性方程为:y=11.363x+93.026,R²=0.9939,LOD为1.4×10^-7mg/mL,测定生活污泥,印染污泥和电镀污泥等3种污泥中的PH,回收率为95.43%～104.26%。更多还原

【Abstract】 In this paper,Lumichrome（LC） as the fluorescent probe was labeled on antibody of Naphthalene（NA） and Phenanthrene（PH）,then the direct competitive immunofluorescence were built to detect the NA and PH.The linear calibration range for the determination of NA was 1.0×10^-7～1×10^-2 mg/mL,y=7.505x+61.54,R²=0.9917, the detection limit was 5.0×10^-8 mg/mL,the recovery of NA in three kinds of soil samples（the living sludge,the plating sludge and the printing sludge） was between 97.53%and 107.57%.The linear calibration range for the determination of PH was 1.0×10^-6～2.0×10^-4 mg/mL,y=11.363x+93.026,the detection limit was 1.4×10^-7 mg/mL,the recovery of PH in three kinds of soil samples was between 95.43%and 104.26%.更多还原