【作者】 曹倩； 赵红； 王健； 丁楠； 向开祥； 何裕建；

【Author】 CAO Qian~1,ZHAO Hong~(*1),WANG Jian~1,DING Nan~1,XIANG Kai-xiang~2 and HE Yu-jian~(*1)(1.College of Chemistry and Chemical Engineering,Graduate University of Chinese Academy of Sciences,Beijing 100049)

【机构】 中国科学院研究生院化学与化学工程学院； 湖南怀化医学高等专科学校；

【摘要】 利用纳米金与单链寡聚核苷酸碱基之间强的亲和力,将单链寡聚胸腺嘧啶脱氧核苷酸吸附于金纳米粒子表面。当加入三聚氰胺后,因三聚氰胺能与胸腺嘧啶脱氧核苷酸发生静电和氢键作用而导致纳米金发生团聚,溶液颜色产生变化,因此可在肉眼水平通过纳米金颜色的变化来识别三聚氰胺分子。更多还原

【Abstract】 We report a simple and sensitive colorimetric detection method for melamine based on oligonucleotides and unmodified gold nanoparticles sensing system.Short oligonucleotides could be adsorbed rapidly on the bare colloidal gold through the exposed bases to keep the well dispersion of gold nanoparticles in water.While in the presence of melamine,melamine might interact with oligonucleotides mainly through electrostatic and hydrogen-bonding interactions, and AuNPs are less well protected thus aggregate,accompanying by color change from red to blue.更多还原