【作者】 郑继红； 张运波； 孙国强； 蒋妍梦； 黄爱琴； 庄松林；

【Author】 Zheng jihong,Zhang yunbo,Sun guoqiang,Jiang yanmeng,Huang aiqing,Zhuang Songlin (School of Optical-electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai,200093,China)

【机构】 上海理工大学光电信息与计算机工程学院；

【摘要】 文章报道了结合频分复用技术的荧光共焦生物显微成像系统的实现原理和方法。通过对双路激发光信号的载频调制,聚焦到生物样品上产生荧光信号,再通过傅立叶变换,滤波和解调制过程,将两路荧光信号还原。频分复用共焦显微成像系统能够实现多通道,实时,快速探测生物细胞荧光信号,能提高荧光共焦显微系统时间和空间分辨率。实验完成了紫外波段激发的双频复用荧光共焦显微成像系统,并成功探测了老鼠神经海马细胞样品发出的双点荧光信号。更多还原

【Abstract】 In this paper,we demonstrated a double frequencies division multiplexed confocal fluorescence (FDMCF) microscopy system using a UV laser as the excitation source.In the double FDMCF system,the laser beam was divided into two arms and each arm was modulated with an individual carrying frequency.These two laser beams were then combined and focused on the target cell (rat neural cell) surface to generate fluorescent signal.Then the fluorescent signals from two points of the rat neural cell surface can be demodulated and distinguished after data processing.Furthermore,a quantitative analysis on the crosstalk among different frequencies was provided as well.The experimental results showed that the FDMCF technology can not only maintain the high spatial resolution,but also realize the multiple points detection synchronously with high temporal resolution (within millisecond level range),which benefits the dynamic studies of living biological cells.更多还原