【作者】 李志惠； 张红漫； 刘建强； 刘朋冲； 赵杰； 李毅； 崔丹； 冯秀莉；

【Author】 LiZhi-hui,ZhangHong-man,LiuJian-qiang,LiuPeng-chong,et. Chest Hospital in HebeiProvince,shijiazhuang 050041,China

【机构】 河北省胸科医院；

【摘要】 目的探讨纤支镜肺泡灌洗液中结核分枝杆菌DNA在肺结核诊断中的价值。方法对187例初治肺结核患者及41例肺炎患者分别应用荧光定量聚合酶链反应(FQ-PCR)技术检测结核分枝杆菌DNA、肺泡灌洗液结核菌培养、纤维支气管镜刷片抗酸染色。结果肺结核患者肺泡灌洗液结核分枝杆菌DNA阳性率为:78.6%、肺泡灌洗液结核菌培养阳性率为28.8%、刷片抗酸染色阳性率为21.9%。肺炎患者肺泡灌洗液结核分枝杆菌DNA、肺泡灌洗液结核菌培养、刷片抗酸染色均阴性。结论荧光定量聚合酶链反应检测肺泡灌洗液结核分枝杆菌DNA,特异性高,用于肺结核诊断可提高肺结核诊断的敏感性和准确性,明显优于纤维支气管镜刷片抗酸染色,亦较肺泡灌洗液结核菌培养诊断灵敏、快速,可作为肺结核诊断较可靠指标。更多还原

【Abstract】 Objective To confer the value of tuberculosis DNA in BAFL Detected by FQ-PCR in Diagnosing Pulmonary Tuberculosis.Methods The BALF of the 187 patients with pulmonary tuberculosis treated first and 41 patients with pneumonia were detected the tuberculosis DNA(TB-DNA)by FQ-PCR and TB culture.The bronchoscopic brushing smears were detected by acid-fast staining.Results Positive acid-fast-bacilli of the patients with pulmonary tuberculosis treated first was78.6%by FQ-PCR,28.8%by TB culture and 21.9%by acid-fast staining.The results were negative in all patients with pneumonia.Conclusion Fluorescence quantitative polymerase chain reaction for detecting the mycobacterium tuberculosis DNA of BALF can be used to improve the sensitivity in diagnosing tuberculosis,is superior than the Acid-fast Staining of Sputum Smear,even more sensitive and rapid than mycobacterium tuberculosis in sputum culture,and it could be used to be a more reliable indicator of TB diagnosis.更多还原