【作者】 杨倩； 王先之； 沈禹颖； 南志标；

【Author】 YANG qian WANG xian-zhi SHEN yu-ying~* NAN Zhi-biao (College of Pastoral Agriculture Science and Technology,Lanzhou University,730020 China)

【机构】 农业部草地农业生态系统学重点开放实验室兰州大学草地农业科技学院；

【摘要】 以黄土高原实施保护性耕作9年的冬麦田土壤为对象,利用BIOLOG方法研究了小麦收获期根际微生物群落功能多样性,结果表明:各耕作处理下微生物利用单一碳源的能力及其代谢多样性指数(群落Shannon和丰富度指数)的顺序为:免耕+秸秆覆盖(nts)和免耕(nt)>传统耕作+秸秆覆盖(ts)>传统耕作(t),传统耕作下的各项指标均低于其他耕作处理,表明免耕有利于土壤微生物活性的提高。糖类是土壤微生物群落利用的主要碳源,免耕处理下根际土壤微生物的特异利用碳源是氨基酸类,耕作处理下则主要为聚合物类碳源。更多还原

【Abstract】 This study investigated the influence of four tillage treatments on rhizosphere microbial diversity by BIOLOG assay on silt soil in the Qingyang Loess plateau.The results show that,the utilization ability of microbial communities under different treatments for single carbon substrates ranked in the order of nts and nt>ts>t.The highest function diversity index,such as Richness index,Shannon index was under nts and lowest under t treatment,conservation tillage increases the microbial activity and community functional diversity of rhizosphere soil in the western Loess Plateau.The principal component analysis indicated that the significant difference between no till and tillage treatments on the utilization ability of microbial communities.Carbohydrates and polymers are the main carbon sources contributed to the differentiation of soil microbial communities from four treatments.更多还原