【作者】 高凤芹； 孙启忠； 邢启明；

【Author】 GAO Feng-qin~(1,2),SUN Qi-zhong~(1,2*),XING Qi-ming~3 (1.Grassland Research Institute,Chinese Academy of Agricultural Sciences,Huhhot 010010;2.Key Laboratory of Grassland Resources and Ecology,Ministry of Agriculture,Hohhot 010010; 3.Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081,China;

【机构】 中国农业科学院草原研究所； 农业部草原资源与生态重点开放实验室； 中国农业科学院研究生院；

【摘要】 本文研究了化学预处理对紫花苜蓿、高丹草、沙打旺和御谷酶解糖化发酵效果的影响,评价4种牧草的能源性。预处理结果表明效果最好的是:40目牧草粉用1%的稀硫酸在100℃预处理60min后,洗至中性,在40～60℃干燥箱烘干。酶解糖化发酵结果表明效果最好的是:加入浓度为3%(w/v)的蛋白胨液,固液比为1:10,121℃灭菌20min后,加入纤维素酶20U/g。采用SSF工艺pH4.8,34℃,100r/min。紫花苜蓿在48h乙醇产量最大达到12.34g/L,乙醇产率为0.41g/g。四种牧草的能源性大小依次为:紫花苜蓿>高丹草>御谷>沙打旺。更多还原

【Abstract】 The effect of pretreatment on enzymatic saccharification of Alfalfa（Medicago sativa L.）,Sorghum hybrid sudan grass（Sorghum bicolor（L.） moench×Sorghum Sudanese（Piper） Stapf）,Erect milkvetch（Astragalus adsurgens Pall.） and Pearl millet（Pennisetum americanum（L.） Leeke） by chemical substances were studied.The result showed the optimum pretreatment conditions as follows:the forage powder of 40 mesh waspretreated in 1%H₂SO₄ at 100℃for 60min,watered to neutral and dried at 40～60℃.The result indicated the optimum enzymatic saccharification and fermentation conditions that was to add cellulose dose 20u/g and 3%（w/v） peptone at 34℃for 48h in the buffer solution of citric acid- citric acid sodium of pH 4.8 and 100r/min,with the ratio of liquid to solid 10.As a result,ethanol concentration reached to maximum value 12.34g/L at 48h,and ethanol production ration was 0.41g/g.position of energy source characteristic was as follows:Alfalfa>Sorghum hybrid sudan grass>Pearl millet>Erect milkvetch.更多还原