【作者】 钟理； 王小利； 干友民； 唐成斌； 尚以顺；

【Author】 Zhong Li~(1,2),Wang Xiao-Li~2,Gan You-Min~1,Tang Cheng-Bin~2,Shang Yi-Shun~2; (1.Department of Grassland Sichuan Agricultural University Ya’an 625014; 2.Guizhou Institute of prataculture Dushan 558200)

【机构】 四川农业大学草业科学系； 贵州省草业研究所；

【摘要】 以国外匍匐剪股颖品种Kromi为对照,采用ISSR分子标记技术,对贵州三大野生匍匐剪股颖居群进行遗传多样性研究,结果表明:9个引物共扩增出多态性带66条,多态性条带比率(PPB)为81.48%,亚居群间遗传相似系数范围在0.4074～0.9123之间,表明贵州野生匍匐剪股颖具有较丰富的遗传多样性;基于Nei’s相似系数对各亚居群进行了聚类分析,地理位置相近的亚居群多可聚为一类,呈现出一定的地域性分布规律,相关分析表明,地理距离与遗传距离显著相关(r=0.494,0.05>P>0.01);居群分化分析结果表明,居群间存在较强的遗传分化,居群间的变异占总变异的49.2%。更多还原

【Abstract】 In a comparison of Kromi,ISSR molecular markers were used to measure the genetic diversity among three populations of wild Agrostis stolonifera L.collected from Guzhou.The results showed:66 polymorphic bands were produced by 9 primers,the percentage of polymorphic bands was 81.48%,and the range of GS was 0.4074～0.9123,it indicated a rich genetic diversity of Agrostis stolonifera L.;Based on the Nei’s genetic distance,subpopulations could be divided into 3 groups,it accorded with Agrostis stolonifera L.’s geography distribution in Guizhou;The analysis of apportioning the variation within and among populations,results indicated 49.2%variation resided among the populations.更多还原