【作者】 关宁； 李聪；

【Author】 GUAN Ning LI Cong (Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094,China)

【机构】 中国农业科学院北京畜牧兽医研究所；

【摘要】 玉米醇溶蛋白(zein)是玉米胚乳中主要的储藏蛋白,富含动物必需的含硫氨基酸—甲硫氨酸和半胱氨酸。试验证明含硫氨基酸对奶制品、肉类及羊毛产量和质量有重要的影响。本研究将含有γ-zein:KDEL基因的质粒pROK.TGILK通过农杆菌介导法转入紫花苜蓿,已获得了19株再生植株,并经过PCR检测证明8株为阳性植株。更多还原

【Abstract】 Zeins,the major protein reserves of maize kernels,are rich in the essential sulphur amino acids methionine and cysteine.It has been demonstrated that sulphur-containing amino acids play an important role in the efficiency of dairy, meat and wool production.The vector pROK.TGILK harboringγ-zein:KDEL gene was transfened into alfalfa mediated by Agrobacterium tumefaciensand 19 transgenic plants were obtained.The result showed that 8 of them had positive signals by PCR.更多还原