【作者】 王丽鸳； 成浩； 曾建明； 周健； 韦康；

【Author】 WANG Li-yuan,CHENG Hao,ZENG Jian-ming,ZHOU Jian,WEI Kang (Tea Research Institute,Chinese Academy of Agricultural Sciences;National Center for Tea Improvement,Hangzhou 310008,China)

【机构】 中国农业科学院茶叶研究所茶树资源与改良研究中心国家茶树改良中心；

【摘要】 序列表达标签(expressed sequence tags,ESTs)为长200～800bp的基因表达序列片段。用EST替代基因组测序,可使研究费用大大降低,尤其是对那些基因组比较复杂的、基因密度比较低的真核生物,EST计划的实施尤其重要。茶树是高度杂合,基因组比较复杂的木本植物,目前基因组测序还为完成,对茶树进行EST测序及生物信息学分析,具有非常重要的理论意义和实际意义。本文对在GenBank中的茶树dbEST数据及相关分子标记开发利用的研究进展进行综述,并对茶树EST的研究存在的问题进行了探讨。更多还原

【Abstract】 Expressed sequence tags(ESTs) are short sub-sequences of a transcribed cDNA sequence of approximately 200～800 base pairs(bp).They therefore represent a low-cost alternative to full genome sequencing.This is of particular value for eukaryote organisms,which tend to have larger and less gene-dense genomes than do prokaryotes.Tea is a highly-hybrid woody plant of complex genome.For present,genome sequencing of tea has not completed,so there are very important theoretical and practical significant by Utilizing EST sequencing and bioinformatics analysis in tea.The dbEST data in the GenBank and the progress of the development or utilization on the molecular markers derived from EST are reviewed in this article. The question exiting in tea EST research are also be explored.更多还原