【作者】 刘敬卫； 黄友谊； 丁建； 刘聪； 肖秀丹； 倪德江；

【机构】 华中农业大学园艺林学学院；

【摘要】 多酚氧化酶是茶树中一种重要酶类,对茶叶产品的品质形成具有重要作用。茶树多酚氧化酶一直是人们的研究重点,但至今未见体外表达茶树PPO。我们在原核中表达纯化获得具有催化活性宜红早PPO的基础上,表达纯化获得宜红早PPO具有催化活性的、切除转移肽的成熟蛋白,同时发现转移肽对PPO活性具有抑制作用。这些结果为茶树PPO工程蛋白在体外大量制备及应用提供了基础。更多还原

【Abstract】 Polyphenol oxdiase (PPO) is a key enzyme in Camellia sinensis for it plays essential roles in quality formation of tea processing. However, there is no report of expressing C. sinensis PPO in vitro, despite that it always is a hotspot. Based on purifying the active Yihongzao PPO with transit-peptide expressed in E. coli, the active mature form was also expressed in E. coli and purified from the inclusion bodies. The activity of the mature form was higher than that of PPO with transit-peptide, which suggests that the transit-peptide should restrain the activity of PPO in vivo and in vitro. It is helpful to yield and apply for the C. sinensis engineering PPO widely.更多还原