【作者】 季清娥； 郭庆亮； 陈家骅；

【Author】 JI Qing-e, GUO Qing-liang,CHEN Jia-hua ( Fujian Agriculture and Forestry University, Fuzhou Fujian, China)

【机构】 福建农林大学益虫研究所；

【摘要】 为系统研究甲基丁香酚(methyl eugenol,ME)对橘小实蝇不育雄虫交配能力的影响,将来自橘小实蝇蛹色伴性遗传品系的羽化前2天的雄蛹,经100Gy的137Cs辐照(剂量率为1.00Gy/min),羽化后的不育雄虫达到10-12日龄时饲喂ME,分别以未饲喂ME的不育雄虫、野生普通种的雄虫作对照,野生普通种的雌虫作交配对象,对不育雄虫交配总次数、连续交配次数、交配竞争能力以及对野生雌虫再次交配发生率等进行了研究。结果表明不育雄虫可多次连续交配,一些雄虫连续交配次数可达到9次,但连续交配总次数、平均单头连续交配次数与对照相比差异不显著(P>0.05);不育雄虫的累积交配次数为344.33±12.55次,单头平均交配次数为6.88±0.25次,与对照无显著差异;饲喂ME的不育雄虫与未饲喂ME的不育雄虫、野生雄虫同时与50头野生雌虫竞争交配时,饲喂ME的50头不育雄虫与50头野生种雄虫竞争时的交配成功率为44.67±2.40%,而未饲喂ME的不育雄虫的交配成功率为22.00±2.31%,二者间差异极显著(t=-6.8,P=0.002,MCI>0.5)。研究表明饲喂ME不能增加不育雄虫交配总次数及连续交配次数,但可以大幅提高不育雄虫的竞争交配能力;同时不育雄虫饲喂ME后对初次交配5日后的雌虫再次交配发生率没有显著影响,但可以提高初次交配10日、15日后雌虫的再次交配发生率。更多还原

【Abstract】 The effect of methyl eugenol(ME)on the total times of mating, consecutive mating, mating competitiveness, multiple mating, and the incidence of wild female remating were studied in sterile males from a genetic sexing strain of Bactrocera dorsalis (Hendel). Male pupae were irradiated at dose of 100Gy by 137 Cs at 2 d before emergence and the dose rate was 1.00Gy/min. Sexually mature 10 day old sterile males were fed ME, while Non-ME-fed sterile males and normal wild males were used as control, and wild females as mating partners. The results showed that some ME-fed sterile males could mate continuously up to nine times, but the total times of consecutive mating and the mean value of continuous mating times were not significant (P> 0.05) compared with the control. The total mating times of ME-fed sterile males was 344.33±12.55 and the mean value was 6.88±0.25, but both have no significant difference compared with the control. The mating success rate of ME-fed and non- ME-fed sterile males mated with wild females were 44.67 ± 2.40% and 22.00 ± 2.31% separately. There were significant differences between them (t=- 6.8, P = 0.002).The outcomes were that feeding on ME did not increase the frequency of multiple mating by sterile males, but significantly increased the mating competitiveness of sterile males against wild males. At the same time, sterile males fed ME did not significantly affect the remating of wild females 5 days after the initial mating, but increased the remating frequency of females 10 and 15 days after the initial mating.更多还原