【作者】 张文斌； 吕振波； 张莹； 陈建强； 李凡； 郑亮； 丛旭日；

【Author】 ZHANG Wen-bin 1,2,LV Zhen-bo 1,ZHANG Ying 1*,CHEN Jian-qiang 1,et al(1.Shandong Marine and Fishery Research Institute,Shandong Provincial Key Laboratory of Restoration for Marine Ecology,Yantai 264006 ;2.Institute of Marine science,Shanghai Ocean University,Shanghai 201306,China)

【机构】 山东省海洋水产研究所,山东省海洋生态修复重点实验室； 上海海洋大学海洋科学学院；

【摘要】 随着养殖规模的扩大和养殖水体生态环境的恶化,水体中的氧气正在逐渐的减少,甚至消失殆尽。本实验采用石蜡切片,用苏木精-伊红(H-E)染色的方法,对菲律宾蛤仔(Ruditapesphilippinarum)缺氧(DO<2mg/L)胁迫20d后组织的病理影响进行研究;同时采用室内生态学方法对菲律宾蛤仔在正常充氧和缺氧状态下的耗氧率、排氨率(NH3-N)、CO2排出率进行了研究。实验结果表明,在石蜡切片中,缺氧组与正常组的菲律宾蛤仔在缺氧20d后有异常,缺氧组组织中分布有零星的深紫色圆斑点。在22℃水温条件下,随着缺氧胁迫时间的推移,缺氧对菲律宾蛤仔耗氧率、排氨率、CO2排出率存在显著性差异。在缺氧第2d时,耗氧率、排氨率和CO2排出率呈下降趋势,分别为0.5665mg/(g·h)、0.0986mg/(g·h)和25.7593mg/(g·h)。耗氧率在第2d达到最低值,为0.5665mg/(g·h);而排氨率、CO2排出率在第20d达到最低值,为0.0891mg/(g·h)和7.4220mg/(g·h),约为对照组的1/2。由此可知,缺氧胁迫对菲律宾蛤仔的生理和能量代谢产生了一定的影响。更多还原

【Abstract】 With the expansion of farming scale and the deterioration of water ecological environment,the oxygen in the water is gradually reduced,or even disappeared.In the experiment,the hematoxylin-eosin stain(H-E) method was employed to detect the pathologic changes of organization in Ruditapes philippinarum exposure to hypoxia(DO <2 mg/L) stress after 20 days.The oxygen consumption,ammonia excretion rate(NH 3-N) and CO2 production were studied in Ruditapes philippinarum exposure to hypoxia(DO <2 mg/L) on the second,5th,10th and 20th day in the indoor ecology method.The results demonstrated that there were some pathologic changes in the gill,hepatopancreas and mantle tissues of Ruditapes philippinarum challenged by hypoxia compared to the tissues in the normal clams.The oxygen consumption,ammonia excretion rate and CO2 production(0.5665 mg/g h,0.0986 mg/g h and 25.7593 mg/g h respectively) were decreased in the clam exposure to hypoxia on the second day.Oxygen consumption reached the lowest level(0.5665 mg/g h) on the second day,is;And ammonia excretion rate and CO2 production on the 20th day reached the lowest contents(0.0891 mg/g h and 7.4220 mg/g h),which were about one half compared to the control group.Therefore,the results indicated that there was a certain influence on the physiological and energy metabolism of clam exposure to hypoxia.更多还原