【作者】 薛淑群； 孙源； 尹洪滨； 孙中武；

【Author】 XUE Shu-Qun 1,2,SUN Yuan 1,YIN Hong-bin 2 and SUN Zhong-wu 1* 1.Life Scientific College,Northeast Forestry University,Harbin 150040,China ;2.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Harbin 150070,China)

【机构】 东北林业大学生命科学学院； 中国水产科学研究院黑龙江水产研究所；

【摘要】 本研究应用ISSR和TRAP标记技术对ZD黄颡鱼性别特异性标记进行筛选。应用ISSR技术获得2个雌性黄颡鱼特异性标记:UBC835-3位点,在雌性黄颡鱼中表达量为93.33%,在雄性黄颡鱼中表达量为13.33%;UBC835-4位点,在雌性黄颡鱼中的表达量为100%,在雄性群体中为0。应用TRAP技术获得2个雄性黄颡鱼特异性标记:ME1-EM1-22位点和ME2-EM3-8位点在雌性黄颡鱼表达量为40%,在雄性黄颡鱼表达量为100%,所以,可将这4个位点作为黄颡鱼性别鉴定的特异性分子标记。更多还原

【Abstract】 In this study,ISSR and TRAP markers were used to screen sex-specific markers of male and female Yellow Catfish(ZD) obtained from Zhaodong Breeding Farm.Results of ISSR-PCR amplification screened two females gender-specific ISSR markers,UBC835-3 sites were seen expressed in female Yellow Catfish(93.33%) but male Yellow Catfish only 13.33%;in contrast,UBC835-4 sites of expression were seen expressed in all male Yellow Catfish,whereas no expression was seen in female Yellow Catfish.Meanwhile,results of TRAP-PCR amplification presented two gender-specific TRAP markers,ME 1-EM 1-22 sites and ME 2-EM 3-8 sites,they were seen expressed in female Yellow Catfish(40%),yet could seen expressed in all male Yellow Catfish.Therefore,these sites can be identified as Yellow Catfish sex-specific molecular markers.更多还原