【作者】 李夸良； 黄捷； 杨国新； 余辉； 潘福生； 吕裕斌； 程元荣；

【Author】 Li Kua-liang~1,Huang Jie~1,Yang Guo-xin~1,Yu Hui~1,Pan Fu-sheng~2, Lv Yu-bin~2,Cheng Yuan-rong~(1*) (1.Fujian Institute of Microbiology,Fuzhou 350007;2.Hangzhou Huadong Medicine Group Biotechnology Institute,Hangzhou 310011)

【机构】 福建省微生物研究所； 杭州华东医药集团生物工程研究所；

【摘要】 从55株小单孢菌转化雷帕霉素的研究中获得了15株能够转化雷帕霉素的小单孢菌。其中,13株小单孢菌转化雷帕霉素产生7个转化产物,它们在HPLC图谱中有相同的相对保留时间。在剩余的2株菌中,我们选出小单孢菌FIM02-848进行深入研究。该菌株转化雷帕霉素产生7个转化产物,我们从转化发酵液中分离获得了其中的两个转化产物。结构鉴定表明,分子量为916的转化产物与14-羟基雷帕霉素同质;HPLC、UV和MS分析均显示分子量为900的转化产物与14-去氧雷帕霉素具有相同的特性,推测它可能是14-去氧雷帕霉素。更多还原

【Abstract】 In the search of microbial conversion of rapamycin by 55 strains of Micromonospora sp.,of 15 strains were found to be capable of transforming rapamycin into various products.To analyse the converted products by HPLC graphs of 15 strains,it showed of 13 strains were with the same relative retention time.1 strain from 2 remainders,Micromonospora sp.FIM02-848 was selected for further study. The strain can transform rapamycin into seven products.Of one converted product(RRT 0.92) with molecular weight of 916 was identified with 14-hydrorapamycin.Of the other converted product with RRT 1.78 showed it was the same with 14-deoxorapamycin in molecular weight of 900,HPLC,UV and MS. Thus,it was inferred to be 14-deoxorapamycin.更多还原