【作者】 范芳； 谢瑞祥； 杨琳；

【机构】 福建省肿瘤医院药理研究室；

【摘要】 目的:建立了一种简单的多西紫杉醇萃取方法和高效的反相高液相色谱法。方法 :血清中多西紫杉醇用乙醚-石油醚(9:1)单步提取;色谱柱:Symmetry RP18(5μm,4.6mm×25mm,waters),流动相:甲醇-乙睛-水=45:22:33。检测波长230nm,室温,内标:地西泮。结果 :多西紫杉醇在0.25-12μg·mL-1范围内呈良好的线性关系(r=0.9993),平均回收率100.11%(n=9,RSD=2.50%)结论:此方法提取步骤少,方法简单快速、准确,适合临床多西紫杉醇的血药浓度监测。更多还原

【Abstract】 OBJECT A simple extraction of docetaxel in serum and a method of HPLC were developed.METHODS: docetaxel was extracted from serum by ethyl ether: petroleum ether(9:1) and analyzed in a column of Symmetry RP18(5μm,4.6mm×25mm,waters) with a mobile buffer of methol-acetonitrile-water(45:22:23) .docetaxel was detected in 230nm with diazepam as internal standard in normal temperature .RESULTS: The linear of docetaxel was excellent within the range of 0.5~12μg·ml-1 (r=0.9993).The average recoverys was 100.11% (n=9,RSD=2.50 %).CONCLUSION : The method is simple,rapid,sensitive and applied to determine the therapeutic drug monitoring of docetaxel.更多还原