【作者】 戴胜； 张明； 程文明； 张勋； 许慧； 潘亚梅； 李俊；

【Author】 DAI Sheng,ZHANG Ming,CHENG Wen-ming ,ZHANG Xun,XU Hui,PAN Ya-mei,LI Jun (School of Pharmacy & Anhui Key Laboratory of Bioactivity of Natural Products & Anhui Research Center of Engineering and Technology for Active Components from Natural Product,Anhui Medical University,Hefei 230032,China)

【机构】 安徽医科大学药学院安徽天然药物活性研究省级重点实验室安徽省天然药物活性成分工程技术研究中心；

【摘要】 目的建立野菊花提取物中3种二咖啡酰奎宁酸的HPLC方法。方法 采用大连依利特HypersilODS2(4.6mm×250mm,5μm)色谱柱;以乙腈(A)-2%醋酸水溶液(B)为流动相,梯度洗脱(0~15min,10%A→15%A;15-25min,15%A→16%A;25-30min,16%A→60%A;30-40min,60%A→10%A);流速1.0mL·min-1;检测波长326nm;柱温35℃。结果 1,3-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸及3,4-二咖啡酰奎宁酸在0.0625-2.0000μg范围内线性关系良好(r>0.9990,n=6);平均回收率(n=6)分别为102.172%,101.120%,100.520%。结论 本方法操作简单,准确性和重现性良好,可以作为同时测定野菊花中3种二咖啡酰奎宁酸含量的方法。更多还原

【Abstract】 OBJECTIVE To establish a HPLC method for determination of three dicaffeoylquinic acids in extracts from Chrysanthemum indicum L.METHODS The separation was carried out on a Elite Hypersil ODS2 ( 4.6 mm × 250 mm,5 μm) colum eluted with mobile phases containing acetonitrile (A)-2% acetic acid solution (B) with gradient mode (0~15 min,10%A→15%A;15-25 min,15%A→16%A;25-30 min,16%A→60%A;30-40 min,60%A→10%A) at the flow rate of 1.0 mL·min-1.The column temperature was 35℃ and the detection wavelength was 326 nm.RESULTS Good linearity between the peak area and injected amount exists when the amount is within 0.0625-2.0000 μg (r > 0.9990,n = 6) for 1,3-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid.The average recoveries (n = 6) were 102.172%,101.120% and 100.520%,respectively.CONCLUSION The method is simple,accurate and repeatable and can be used for the determination of three dicaffeoylquinic acids simultaneously in Chrysanthemum indicum L.更多还原