【作者】 文雪； 周鑫； 崔钟铉； 赵丽纯；

【机构】 吉林大学药学院；

【摘要】 目的:构建EphB4胞外段基因的原核表达载体,获得重组可溶性EPHB4胞外段多肽。方法:利用RT-PCR方法扩增EphB4基因片段,与PSJ-3载体连接,构建重组质粒PSJ-3/EphB4,转化大肠杆菌BL21(DE3)并进行诱导表达。结果:经酶切及DNA序列测定证实PSJ-3/EphB4中的EphB4基因正向插入、序列正确,并在BL21(DE3)中获得稳定表达。结论:本实验成功构建了PSJ-3/EphB4重组质粒并获得稳定表达,为进一步研究可溶性EPHB4胞外段多肽对恶性肿瘤的影响奠定基础。更多还原

【Abstract】 Objective:To construct the recombinant plasmid PSJ-3/EphB4 and obtain recombinant soluble EPHB4 protein.Methods:EphB4 gene fragment was amplified by RT-PCR and ligated with PSJ-3 vector to construct PSJ-3/EphB4 recombinant plasmid.Then it was transformed into E.coli BL21(DE3) and induced to express the EPHB4 proteins.Results:EphB4 gene was positively inserted PSJ-3 and the sequence was right,and it could express in BL21(DE3).Conclusion:the PSJ-3/EphB4 is successfully constructed and could express the EPHB4 protein.It lay a basis for further evaluating the effect of soluble EPHB4 on cancer.更多还原