【作者】 徐红燕； 汤湛； 王俏； 张望刚；

【Author】 XU Hong-yan,TANG Zhan,WANG Qiao ,ZHANG Wang-gang(Institute of Materia Medica,Zhejiang Academy of Medical Sciences,Hangzhou,Zhejiang 310013,China)

【机构】 浙江省医学科学院药物研究所；

【摘要】 目的研究微透析探针回收率与流速、药物浓度等之间的关系。方法以川芎嗪为检测药物,测定不同流速、不同药物浓度时,体外正、反透析法的探针回收率,以及体内反透析法的探针回收率。结果探针回收率与流速成反比,与川芎嗪浓度无关,体外正向与反向两种透析法得到的探针回收率差异较大,而体外正向透析法与体内反向透析法得到的探针回收率比较接近。结论药动学研究中,可采用反透析法测得的体内回收率作为药物的探针回收率。更多还原

【Abstract】 OBJECTIVE To evaluate the effects of flow rates and concentrations on the recoveries of microdialysis probes.METHODS Tetramethylpyrazine(TMP) was selected as model drug.in vitro recovery of TMP was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations.And in vivo recovery of TMP was studied using retrodialysis methods.RESULTS The microdialysis recoveries of TMP were inversely proportional to the perfusing rates,independent of TMP concentrations.The recoveries of positive dialysis were quite different from that of retrodialysis in vitro.While in vitro positive dialysis recovery was similar to in vivo retrodialysis recovery.CONCLUSION Microdialysis is a useful and reliable tool to study the pharmacokinetics of TMP and in vivo retrodialysis method can be used for TMP pharmacokinetics.更多还原