【作者】 詹勤； 高守红； 陈万生；

【Author】 QIN Zhan,SHOUHONG Gao,WANSHENG Chen Department of Pharmacy,Changzheng Hospital,Second Military Medical University,No.415,Fengyang Road,Shanghai 200003,China;

【机构】 第二军医大学长征医院药学部；

【摘要】 目的:建立大鼠血浆中桔梗皂苷D的LC-MS/MS分析方法,分析桔梗皂苷D的药代动力学特征;分别给予桔梗皂苷D及桔梗提取物,评价桔梗皂苷D在大鼠体内的生物利用度。方法:液相部分,采用AgilentC18柱(100mm×2.1mmi.d.,3.5μm),流动相A相为60%甲醇,B相为40%含0.1%甲酸的水溶液,流速为0.25ml/min;质谱部分,采用电喷雾-三重四极杆质谱仪,负离子条件下多反应监测模式。结果:桔梗皂苷D在5-2000ng/ml范围内线性良好(r2>0.99),最低定量限为5ng/ml;成功应用该法获得桔梗皂苷D在大鼠体内的药代动力学参数及口服生物利用度。结论:该法简单、快速、灵敏,适宜检测大鼠血浆中桔梗皂苷D的浓度;大鼠灌胃桔梗提取物时,桔梗皂苷D的生物利用度高于单独给予桔梗皂苷D。更多还原

【Abstract】 OBJETIVE:Develop a HPLC-MS/MS method for the quantification of platycodin D(PD) in rat plasma and apply it to gain the main pharmacokinetic parameters of PD after oral administration of PD alone and Platycodi Radix extract.METHODS:Ginsenoside Rg1 was used as the internal standard(IS).After simple protein precipitation of the plasma samples with methanol,the analytes were separated on an ODS column(100mm×2.1mm i.d.,3.5μm) with the mobile phase of methanol-water(60:40,v/v) containing 0.1% formic acid with a flow rate of 0.25mL/min.The detection was performed on a triple quadruple tandem mass spectrometry in the negative multiple reaction monitoring(MRM) mode via electrospray ionization(ESI) source with a chromatographic run time of 4.0min.RESULTS:The calibration curve was linear from 5 to 2000ng/mL(r2>0.99) with a lower limit of quantification(LLOQ) of 5ng/mL.The intra-and inter-day precision(relative standard deviation,RSD) values were below 15% and the accuracy(relative error,RE) was from-15% to +15% at three quality control(QC) levels.CONCLUSION:The method was successfully applied to assess the pharmacokinetics and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract.更多还原