【作者】 丁愉； 汤海峰； 程光；

【Author】 DING Yu1,TANG Hai-feng1,CHENG Guang2(1.Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Xi’an 710032;2.Department of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi’an 710032)

【机构】 第四军医大学西京医院药剂科； 第四军医大学西京医院脑科医院；

【摘要】 目的首次研究小花草玉梅的化学成分。方法应用多种色谱手段和波谱方法分离和鉴定化合物。采用MTT法测定肿瘤细胞毒性。结果分离鉴定了15个化合物(1～15),包括1个半日花烷型二萜苷类化合物(13)、1个四氢呋喃型木脂素苷类化合物(14)、1个三萜苷元(15)和12个齐墩果烷型三萜皂苷(1～12);所有化合物均为首次从本种得到,10,11,13和14首次从银莲花属植物中得到且10,13和14为3个新化合物。皂苷5和12对BEL-7402和HeLa肿瘤细胞显示显著细胞毒性。结论首次从银莲花属植物发现二萜苷和木脂素苷两种类型的化合物,首次从该属植物发现具有天然届少见的丝石竹皂苷元的三萜皂苷(10),表明本种作为一个变种的特殊性。首次从化学成分角度验证了本种属于银莲花属植物。本研究填补了对小花草玉梅化学成分的研究空白,为开发这一在我国拥有丰富蕴藏量的药用资源提供了科学依据。更多还原

【Abstract】 OBJECTIVE To study the chemical constituents from the Anemone rivularis var.flore-minore for the first time.METHODS A combination of multi-chromatography as well as spectral analysis were employed for the separation and determination of the compounds from the plant.The cytotoxicities against human tumor cells were determined using the MTT assays.RESULTS Fifteen compounds(1-15),including one labdane type diterpenoid glycoside(13),one tetrahydrofuran type lignan glycoside(14),one triterpene sapogenin(15) and twelve triterpenoid saponins(1-12) were obtained and elucidated from the plant for the first time,among which four were found in genus Anemone for the first time(10,11,13 and 14) and three are new compounds(10,13 and 14).Saponins 5 and 12 showed in vitro cytotoxicity against human BEL-7402 and HeLa tumor cells.CONCLUSION Diterpenoid glycoside and lignan glycoside were found in genus Anemone for the first time,Saponin 10 possessed gypsogenin as aglycone,which was first found in genus Anemone,too.All these support that the title plant is a variation from Anemone rivularis.A.rivularis var.flore-minore was first proved to belong to genus Anemone from the chemical taxonomic point of view.Our studies established a homebase for further development of the title medicinal plant with abundant resources in China.更多还原