【作者】 张蕾； 严萍； 詹若挺； 尹丹丽； 李璐； 梁小银； 陈蔚文；

【Author】 ZHANG Lei,YAN Ping,ZHAN Ruoting,YIN Dangli,LI Lu,LIANG Xiaoyin,CHEN Weiwen Research Center of Chinese Medicinal Resource Science and Engineering,Guangzhou University of Traditional Chinese Medicine、Key Laboratory of Chinese Medicine Resources of Ministry of Education, Guangzhou 510006,China

【机构】 广州中医药大学中药资源科学与工程研究中心、教育部(省部共建)中药资源科学重点实验室；

【摘要】 白及Bletilla striata(Thunb.)Reichb.f.为兰科植物白及属植物,具有收敛止血,消肿生肌之功效。白及鳞茎组织中含40%～50%天然水溶性多糖,俗称白及胶,其主要化学成分是葡萄甘露聚糖。多糖的提取方法有多种,包括回流浸提,超声波提取和微波提取等方法。目前白及多糖的提取多用回流浸提法,而微波辅助法提取白及多糖尚未见有研究报道。本试验采用微波辅助提取技术,以多糖得率为评价指标,通过单因素试验和正交试验,探讨料液比、提取温度、提取时间和提取次数对白及多糖提取率的影响,优化提取方法。微波辅助提取白及多糖的最佳条件为:按料液比1:75(W/V,g:mL),以提取温度50℃,提取时间为15 min,微波功率700 W,提取2次。在此微波辅助提取条件下,白及多糖的提取率为48.09%,优于超声波提取率(36.71%)及回流提取率(42.11%)。各因素对白及多糖影响的主次顺序为:提取时间>提取次数>料液比>提取温度。该微波辅助方法与传统的回流浸提法相比,具有节能高效的优点。该研究为进一步的白及多糖生物活性研究打下基础。更多还原

【Abstract】 Objective To develop a Microwave-assisted exaction method of Polysaccharide from Bletilla striata.Methods Single factor method coupled with orthogonal array design were used to present the effect of the extraction process.Results The results showed that the optimal process was determined as follows:Liquid/material ratio was 75:1(W/V,g:mL) and the power of microwave was 700w.The material was extracted 2 times with 50℃(15 min each time).The order of the effects of the factors was extraction time>extraction times>liquid/material ratio>temperature. Conclusion In comparison with conventional reflux extraction,microwave-assisted extraction had the benefits of saving energy,high productivity.更多还原