【作者】 陈锡强； 刘可春； 韩利文； 何秋霞； 袁延强； 侯海荣； 王希敏；

【Author】 Chen Xiqiang1.2,Hou Hairong1.2,Wang Ximin1.2,Liu Kechun1.2,Han Liwen1.2,,He Qiuxia1.2 ,Yuan Yanqiang1.2 . (1.Biology Institute of Shandong Academy of Sciences,Jinan 250014,china 2.Research and Development Platform for Drug Screening of Shandong Academy of Sciences)

【机构】 山东省生物传感器重点实验室； 山东省科学院药物筛选技术研发平台；

【摘要】 目的:观察PTK787对转基因荧光斑马鱼的节间血管生成的影响方法采用荧光显像的方法考察多个浓度PTK787对斑马鱼节间血管生成的抑制率,并对各浓度下各组胚胎的毒性反应进行考察。结果0.25μg·ml-1以上浓度都具有明显的抑制血管生成的作用,使36hpf后斑马鱼节间血管生成受到明显抑制。同时在0.25μg·ml-1以上有心脏囊肿、心脏线性化、翘尾发生。结论:PTK787可以有效的抑制转基因斑马鱼节间血管形成,同时具有致畸作用。更多还原

【Abstract】 Objective To explore the effect of tyrosine kinase inhibitor PTK787 on angiogenesis of transgenic zebrafish, whose blood vessel is marked by green fluorescent protein. Methods The number of intersegmental blood vessel and the toxicity of PTK787 were detected by use of fluorescent microscope after the zebrafish was treated with or without PTK787. Results The angiogenesis of intersegmental blood vessel was obviously inhibited after the zebrafish was incubated with more than 0.25μg·ml-1 PTK787 for 12 h (36 hpf), which was accompanied with pericardial oedema, abnormal in heart development and tail rising. Conclusion PTK787 significantly inhibited the angiogenesis of transgenic zebrafish and it might restrain the growth of tumor.更多还原