【作者】 刘旭阳； 李宏霞； 王莉； 陈伟伟；

【Author】 Xuyang Liu1,Hongxia Li2,Li Wang2,Weiwei Chen1 1 Department of Ophthalmology and Ophthalmic Laboratories,West China Hospital,Sichuan University,China (610041) 2 West China-Frontier PharmaTech(National Chengdu Center For Safety Evaluation of Drugs)

【机构】 四川大学华西医院眼科中心·眼科学研究室·韦素眼科研究所； 成都华西海圻医药科技有限公司(国家成都中药安全性评价中心)；

【摘要】 目的:初步探讨碘酸钠诱导视网膜变性的作用机制及非促分裂人酸性成纤维细胞生长因子(non-mitogenetic human acidic fibroblast growth factor,nm-haFGF)对碘酸钠所致大鼠视网膜变性的影响。方法:从SD大鼠的尾静脉注入碘酸钠(50mg·kg-1)建立视网膜变性模型;1h后大鼠右眼玻璃体注射nm-haFGF(0.25mg·ml-1)10μl为给药组,左眼注射等量生理盐水作为对照。分别于1、7、21天行闪光视网膜电图检查,于3、7、14、28天处死动物,取眼球作病理观察。结果:碘酸钠注射后两组大鼠ERG的a、b波幅均出现明显下降,注射前及注射后第3天,两组之间振幅无显著差异;注射7天后,nm-haFGF组下降幅度小于生理盐水组(P<0.01)。病理显示损伤由碘酸钠注射后第7天始出现,表现为光感受器外段结构破坏,出现空泡样改变;第14天,视网膜色素上皮细胞增生,排列不规则,光感受器层崩解,内外颗粒层排列不规则;第28天,视网膜外层失去正常结构,伴有明显的增殖团块,内外颗粒层排列紊乱。同时期nm-haFGF组组织损伤明显小于对照组。结论:本研究首次证实了nm-haFGF能有效减轻碘酸钠所致的大鼠视网膜功能及结构损伤,提示nm-haFGF有一定的神经保护作用。更多还原

【Abstract】 Purpose:To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinaldegeneration induced by NaIO3 in rats. Methods:Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1% NaIO3 at 50mg.kg-1. One hour after NaIO3 treatment, the right eyes received intravitreal injection of 2.5 μg nm-haFGF in 10μL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination. Results:A model of retinal degeneration in rat was established successfully using NaIO3 injection. Signifi cant decreases in both ERG a- and b-wave amplitudes were detected in NaIO3-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO3-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P <0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes. Conclusions:Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO3.更多还原