【作者】 刘杰模； 李先文；

【Author】 Liu Jiemo,Li Xianwen,(Science School,Guangdong Ocean University,Zhanjiang 524088,P.R. China)

【机构】 广东海洋大学理学院；

【摘要】 我国水产品产量目前居世界第一位,有着丰富的水产资源,近年来以鱼虾贝等水产品为原料制备ACEIP成为热点研究领域之一。因为随着经济的增长,高血压的发病率在我国也逐年增高。在高血压患者中,已发现血压升高的占25%,接受治疗的只占12%,而血压得到有效控制的仅有3%,我国高血压的防治工作任重而道远。本实验以罗非鱼下脚料为原料,采用酶水解法制备降血压肽。以ACE抑制率为指标,利用响应面法对酶解下脚料制备ACE抑制肽的工艺条件进行优化,在酶解条件(加酶量、液固比、酶解时间)和ACE抑制率之间建立了数学模型Y=65.5847-1.72820X1+0.8387X2-5.24465X22-2.62694X3。优化后的工艺参数为:加酶量1.91%、液固比3.01、酶解时间2.65h。根据回归方程的预测结果,其ACE抑制率达67.4%更多还原

【Abstract】 Objective At present, China’s aquatic products ranking first in the world, is rich in aquatic resources, in recent years in order to fish for shellfish and other aquatic ACEIP Preparation of raw materials has become one of hot research areas. Because with the economic growth, the incidence of hypertension in China has increased year by year. In patients with high blood pressure, elevated blood pressure have been found 25% of the total treatment and only 12% effective control of blood pressure and only 3%, China’s prevention and treatment of high blood pressure a long way to go. Methods Tilapia in this experiment in order to waste as raw material, the use of enzymatic hydrolysis peptides alternate legal system. Results To ACE inhibition rate as an index, using response surface method for enzymatic preparation of ACE inhibitory peptide scraps of process conditions to optimize the conditions for the enzyme (enzyme volume, liquid-solid ratio, digestion time) and between ACE inhibition rate the mathematical model Y = 65.5847-1.72820X1 +0.8387 X2-5.24465X22-2.62694X3. Conclusion Optimized process parameters are:1.91 percent increase in enzyme capacity, liquid-solid ratio 3.01, enzymolysis time 2.65h. According to the forecast results of the regression equation, the ACE inhibition rate of 67.4%更多还原