【作者】 熊文说； 孙洋； 张杨； 颜剑波； 王鸿； 刘化章； 应国清；

【Author】 XIONG Wen-yue1,SUN Yang1,ZHANG Yang1,YAN Jian-bo3,LIU Hua-zhang2,YING Guo-qing1 (1. College of Pharmaceutical Science,Zhejiang University of technology,Hangzhou,310014,China; 2. College of Chemical Engineering and Materials Science,Zhejiang University of technology,Hangzhou,310014,China; 3. Zhejiang New-Dankong Pharmaceutical CO.,LTD,TaiZhou 318000,China )

【机构】 浙江工业大学药学院； 浙江新东港药业股份有限公司； 浙江工业大学化工与材料学院；

【摘要】 目的制备水溶性壳聚糖衍生物。方法利用壳聚糖C2位上的活泼氨基与还原性酮糖果糖进行缩合反应,合成了N-果糖壳聚糖Schiff碱(CS-D-fructosend,n=天数),并进一步用NaBH4将Schiff碱还原得到N-果糖壳聚糖(CS-D-fructosenr,n=天数)。对N-果糖壳聚糖Schiff碱和N-果糖壳聚糖进行了红外表征并测定了溶解度和清除DPPH自由基的能力。结果 67oC反应5天的N-果糖壳聚糖Schiff碱的溶解度最大,达到20.3g·L-1。N-果糖壳聚糖Schiff碱对DPPH的半清除浓度(EC50)为20μg·mL-1,N-果糖壳聚糖的半清除浓度约为30μg·mL-1。结论 N-果糖壳聚糖Schiff碱还原后水溶性都有不同程度的降低。N-果糖壳聚糖Schiff碱和N-果糖壳聚糖对DPPH自由基均具有较强的清除能力,前者高于后者。更多还原

【Abstract】 OBJECTIVE To prepare water-soluble chitosan derivatives. METHODS Facile conversions can be achieved by specific attachment of D-fructose to the 2-amino functions of chitosan or further reductive alkylation of Schiff bases using sodium borohydride. The structures of the derivatives were characterized by FTIR. Their antioxidant activities were evaluated by the scavenging of 1,1-diphenyl-2-picrylhrazyl radical (DPPH) radical. RESULTS The chitosan-fructose derivative reacting five days was highest at 20.3 g·L-1. The EC50 of Schiff base-type derivative was 20 μg·mL-1, and the EC50 of reduced derivative was 30 μg·mL-1. CONCLUSION The solubilities became worse after reducing. Schiff base-type derivatives had better antioxidant activity.更多还原