【作者】 关心； 吴丽霞； 赵玉红； 康宁； 平静； 王晓蕾； 孙超； 李坚； 石磊；

【Author】 Guan xin1, Wu lixia1, Zhao yuhong1, Kang ning 2, Ping jing2, Wang xiaolei1, Sun chao1, Li jian1, Shi lei1. 1.Shenzhou Hospital affiliated Shenyang Medical College, Shenyang 110002; 2.Shenyang Pharmaceutical University, Shenyang 110016

【机构】 沈阳医学院沈洲医院； 沈阳药科大学；

【摘要】 目的:本研究着眼于糖尿病生理上的必然联系,即体内葡萄糖有氧氧化代谢三羧酸循环(TAC)阶段这一糖代谢必经通路中关键限速步骤代谢酶浓度和生化代谢谱变化。方法:选择符合WHO糖尿病诊断标准病例56例,分成5组:健康人组、糖尿病高危人组、糖尿病前期即糖调节受损组、糖尿病早期组和糖尿病中后期组。Elisa法测定糖代谢关键酶:柠檬酸合成酶、异柠檬酸脱氢酶、α-酮戊二酸脱氢酶,磁微粒分离免疫分析法测定胰岛功能(C-肽),微粒子化学发光分析仪测定胰岛素浓度,生化分析法测定血糖(Glu)、血脂(TC、TG、HDL-C、LDL-C)、肝肾功能、游离脂肪酸(FFA)等生化指标。结果:各观察组异柠檬酸脱氢酶(NAD-ICD)活性均低于对照组,高危组和糖调节受损组之间无显著性差异且活性最低,糖尿病早期组和中后期组NAD-ICD活性略有增强,但还低于对照组。结论:NAD-ICD是TCA循环中的关键限速酶,它的活性影响了TAC的代谢流量。更多还原

【Abstract】 Objective: This study focuses on inevitable connection of diabetes mellitus(DM) physiology, that is, the metabolic enzymes levels and biochemical changes in metabolic spectrum at the key rate-limiting steps of tricarboxylic acid circulation (TAC), a necessary metabolism pathway of glucose aerobic oxidation in the body. Method: 56 cases were selected and divided into 5 groups: healthy control group, DM liability group, impaired glucose regulation (IGR) group, earlier period DM group, mid and later period DM group. The blood citrate lyase, isocitrate dehydrogenase, α-ketoglutaric dehydrogenase were analyzed by Elisa method. The blood glucose, Insulin and C-peptide levels were determined by enzymatic oxidation, magnetic antibody immunoassay (MAIA) methods. The blood lipid, heart function, liver and renal functions were also analyzed by biochemical methods. Results: In liability group and IGR group the isocitrate dehydrogenase (NAD-ICD) activities were significantly lower than that in the control group (p<0.01), in earlier period DM group, the mid and later DM group the NAD-ICD activities slightly increased, but lower than the control group(p<0.05). Conclusions: NAD-ICD is the TAC key rate-limiting enzyme that affects the metabolism of TAC velocity of flow.更多还原