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山银花中绿原酸的提取与含量测定

Extraction and Determination of Chlorogenic Acid in Lonicera confusa

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【作者】 王小平何军刘露郑兴刘运美

【Author】 WANG Xiao-ping1#, HE Jun2*, LIU Lu , ZHENG Xing1, LIU Yun-mei1 (1The institute of Pharmacy&Pharmacology , University of South China , Hengyang 421001, China 2 Department of Chemistry and Chemical Engineering , University of South China , Hengyang 421001, China)

【机构】 南华大学药物药理研究所南华大学化学化工学院

【摘要】 目的比较水提醇沉法及超滤法对山银花中绿原酸提取率的影响。方法采用水提醇沉法及超滤法对山银花中绿原酸进行提取,用紫外分光光度法测定绿原酸含量。结果超滤法提取绿原酸含量达到7.94%。结论山银花中绿原酸的提取以水提75%乙醇沉淀为佳,对绿原酸提取物的精制超滤法更好。

【Abstract】 OBJECTIVE Comparing the impact of water extract-alcohol precipitation and ultrafiltration on extraction rate of chlorogemic acid in Lonicera Confusa. METHODS Adopting the water extract-alcohol precipitation and ultrafiltration to extract the chlorogemic acid in Lomicera Confusa, the chlorogemic acid contents was determined by Ultraviolet Spectrophotometry. RESULTS The chlorogemic acid contents was 7.94% by ultrafiltration, it was the highest. CONCLUSIONS Water extract-75%alcohol precipitation was more effective in extraction technology of chlorogenic acid in Lonicera Confusa, ultrafiltration was profit to refine chlorogenic acid.

【关键词】 山银花绿原酸提取含量测定
【Key words】 Lonicera Confusachlorogenic acidextractiondetermination
  • 【会议录名称】 2009年中国药学大会暨第九届中国药师周论文集
  • 【会议名称】2009年中国药学大会暨第九届中国药师周
  • 【会议时间】2009-11-21
  • 【会议地点】中国湖南长沙
  • 【分类号】R284
  • 【主办单位】中国药学会(Chinese Pharmaceutical Association)、湖南省人民政府
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