【作者】 冯瑞儿； 潘竞锵； 刘广南； 邝少松； 黄小琼； 饶子亮；

【Author】 FENG Rui-er1, PAN Jing-qiang2, LIU Guang-nan2, KUANG Shao-song3, HUANG Xiao-qiong3, RAO Zi-liang3 (1Dept. of ECG, The Second Affilliated Hospital of Guangzhou Medical College, Guangzhou 510260, China; 2 Guangzhou Institute of Traditional Chinese Medicine & Materia Medica,Guangzhou, 510130, China; 3 Guangdong Medical Experimental Animal Center, Foshan 528248 )

【机构】 广州医学院第二附属医院； 广州市中医中药研究所； 广东省医学实验动物中心；

【摘要】 目的:观察益肾I方对多囊卵巢综合征(PCOS)大鼠胰岛素抵抗(IR)、血脂异常及氧化应激-自由基损伤的作用。方法:采用十一酸睾酮加绒促性素(HCG)建立PCOS大鼠模型,连续口服给予二甲双胍或不同剂量的益肾I方4周,观察大鼠体重、血清肿瘤坏死因子-α(TNF-α)、睾酮(T)、丙二醛(MDA)、一氧化氮(NO)、胆固醇(TC)、甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)、空腹血糖(FBG)和口服葡萄糖后2小时血糖(OGTT-2hBG)和胰岛素含量以及超氧化物歧化酶(SOD)和诱导型一氧化氮合酶(iNOS)-一氧化氮合酶(NOS)活性,并计算胰岛素敏感指数(ISI)的变化。结果:PCOS大鼠体重明显增加,与空白对照组比较,差异有高度显著性(P<0.01);血清TNF-α,T,MDA,NO,TG,TC,LDL-C,FBG,OGTT-2hBG和胰岛素水平以及iNOS-NOS活性,均明显升高,而HDL-C含量、SOD活性和ISI,则显著降低,与空白对照组比较,差异有高度显著性(P<0.01);二甲双胍和益肾I方高和中2个剂量均能不同程度地改善PCOS大鼠体重增加、高雄激素血症、高血糖血症、高脂血症、高胰岛素血症和胰岛素抵抗等病理生理改变(P<0.01~0.05),以及增强抗氧化酶SOD活性,降低MDA和NO水平,抑制NOS和iNOS活性(P<0.01~0.05)。结论:益肾I方能改善PCOS大鼠胰岛素抵抗、高雄激素血症和脂质代谢异常,以及对抗氧化应激-自由基损伤,并提高抗氧化能力。更多还原

【Abstract】 Objective To investigate Yishenyifang (called YSYF for short in the following) on insulin resistance, blood glucose-lipid abnormalities and free radical injury in rats with polycystic ovary syndrome (PCOS) and metabolic syndrome (MetS). Method The model of PCOS-MetS rats induced by testosterone undecanoate and HCG subcutaneously (sc) injected in different period, as well as the blank control group sc injected ministration of solvent; afterwards, 70 rats were randomly divided into 5 groups according to body weight : there were 14 rats in model group of PCOS-MetS, 42 in experimental drug groups which were given by YSYF 25 g·kg-1, 12.5g·kg-1 and 6.25g·kg-1, 14 in positive control group by the administration of metformin (0.25g·kg-1), as well as 14 in the blank control group; then all drugs or solvent were adiministered respectively by intragastric administration (ig), qd for 6 weeks. The end of the experiment, the body weight was measured, the contents of fasting blood-glucose (FBG), 2-hours blood glucose after oral glucose tolerance test (OGTT-2hBG), insulin (Ins), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), tumor necrosis factor (TNF)-α, testosterone (T), malondialdehyde (MDA), nitric oxide (NO) were determined, and activities of inducible nitric oxide synthase (iNOS), nitric oxide synthase (NOS) and superoxide dismutase activity (SOD) were tested, and the change of insulin sensitivity index (ISI) was counted. Results Compared with the normal group, body weight, FBG, OGTT-2hBG, Ins, TC, TG, LDL-C, TNF-α, T, MDA, NO and iNOS were increased significantly (P<0.01), but ISI, HDL-C and SOD were decreased obviously (P<0.01). All groups of metformin and YSYF high and middle dosages could reduce the levels of FBG, OGTT-2hBG, Ins, TC, LDL-C, TG, TNF-α, T, MDA, NO and activities of iNOS and NOS, and improve activity of SOD, increase Concentration of HDL-C and enhance ISI, etc in PCOS-MetS rats (P<0.01 or P<0.05). Conclusion Yishenyifang and metformin can significantly improve the insulin senfitivity and ameliorate blood glucose and lipid metabolism and antagonize pathologic changes of dyslipidemia, hyperinsulinemia, hyperandrogenemia and free radical injury in PCOS-MetS rats.更多还原