【作者】 王瑞； 石燕红； 赵森淼； 谭红胜； 侴桂新； 王峥涛；

【Author】 WANG Rui~(1,2),SHI Yan-hong~1,ZHAO Sen-miao~(1,2),TAN Hong-sheng~1,CHOU Gui-xin~(1,2), WANG Zheng-tao~(1,2*) (1.Shanghai University of Traditional Chinese Medicines,Shanghai 201203,China;2.Shanghai R&D Center for Standardization of Traditional Chinese Medicines,Shanghai 201203,China)

【机构】 上海中医药大学中药学院中药研究所教育部中药标准化重点实验室； 上海中药标准化研究中心；

【摘要】 目的建立同时测定独活中蛇床子素和二氢欧山芹醇当归酸酯含量的反相高效液相色谱法。方法Shiseido C₁₈ MG（250mm×4.6mm,5μm）色谱柱,流动相乙腈-水（49:51）,检测波长330nm,流速1.0mL·min^-1,柱温30℃。结果蛇床子素在0.0082～0.656 mg·mL^-1内呈良好线性,r=1,平均回收率99.6%（RSD=1.9%）;二氢欧山芹醇当归酸酯在0.0036～1.536 mg·mL^-1内呈良好线性,r=0.999 9,平均回收率99.4%（RSD=2.0%）。结论通过对22批不同来源独活药材与饮片的含量测定,证明方法简单,准确可靠,重复性好。在重庆巫山地区以海拔1600～1700 m、向阳、黑土的生长条件下,独活中蛇床子素和二氢欧山芹醇当归酸酯的含量均较高。更多还原

【Abstract】 OBJECTIVE To develop a quantitative reversed phase high performance liquid chromatography method for simultaneous determination of osthole and columbianadin in Radix angelicae pubescentis.METHODS The samples were separated at 30℃on a Shiseido C₁₈ MG（5μm,4.6 mm×250 mm） column eluted with acetonitrile-water（49:51） as the mobile phase.Flow rate was set at 1.0 mL·min^-1 and the detection wavelength was set at 330 nm.RESULTS The calibration curve of osthole was linear within the range from 0.0082 to 0.656 mg·mL^-1（r =1） and the average recovery was 99.6%（RSD=1.9%）. The calibration curve of columbianadin was linear within the range from 0.0036 to 1.536 mg·mL^-1（r=0.999 9） and the average recovery was 99.4%（RSD=2.0%）.CONCLUTION The developed method was proved to be simple,reliable and useful by analysis of 22 batches of the samples purchased from different areas.The samples collected in 1600～1700 altitude,abundant light supply and blackland accumulate much higher amount of osthole and columbianadin than those of other growing environments of Mt.Wu.更多还原