【作者】 孟佳； 徐彩菊； 傅剑云； 鹿伟； 陈玉满； 章荣华；

【Author】 MENG Jia,XU Cai-ju,FU Jian-yun,LU Wei,CHEN Yu-man,ZHANG Rong-hua(Zhejiang Centre for Disease Control ang Prevention,Hangzhou 310051,China)

【机构】 浙江省疾病预防控制中心；

【摘要】 目的:研究褪黑素对小鼠睡眠的改善作用,并确定其产生效应较合适的剂量。方法:选用健康雌性ICR小鼠每天经口灌胃给予褪黑素,观察直接睡眠作用,连续给予受试物30d后进行延长戊巴比妥钠睡眠时间试验、戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验。结果:褪黑素剂量达到0.231mg/kg体重时,可与巴比妥钠协同作用缩短小鼠的睡眠潜伏期;褪黑素剂量达到0.462和1.386mg/kg体重时不仅能与巴比妥钠协同作用缩短小鼠的睡眠潜伏期,还能与戊巴比妥钠协同作用增加入睡小鼠的个数,具有提高入睡率的作用。结论:褪黑素能缩短小鼠的睡眠潜伏期,并能增加入睡动物个数,且以0.462mg/kg体重的剂量较为合适。更多还原

【Abstract】 Objective:To study the sleep improvement effect and the optimal dose of the Melatonin in mice.Methods:Healthy ICR female mice were gavaged with the melatonin everyday,and the direct sleep effect of the test material was observed. 30 days later,the sleep extending test with Sodium Pentobarbital,the sleep-producing test with subthreshold dose of Sodium Pentobarbital and the incubation period test with Sodium Pentobarbital were conducted.Results:The melatonin could significantly shorten the incubation period induced by Sodium Pentobarbital in the dose of 0.231,0.462 and 1.386mg/kgB.W.,and significantly raised the numbers of the sleeping mice and the sleep rate.Conclusion:The melatonin could improve sleep of mice and its optimal dose was 0.462 mg/kgB.W.更多还原