【作者】 金虹； 李凤； 曾彬；

【Author】 JIN Hong,LI Feng (College of life Science and Engineering,Southwest University of Science and Technology,Miangyang 621010,China)

【机构】 四川中医药高等专科学校； 西南科技大学生命科学与工程学院；

【摘要】 目的对四川平武天麻进行以天麻素和天麻多糖为标志性功效成分的提取制备工艺及其含量控制的研究。方法采用乙醇回流法、超声法进行了以天麻素和天麻多糖为标志性的功效成分提取工艺的研究,以薄层层析法对天麻素进行定性及半定量检测,并用经紫外分光光度法和标准曲线法分别测定其中天麻素和天麻多糖含量。结果乙醇连续回流法(Soxhlet)提取天麻素效果较好;正交试验表明较优的提取条件为浓度70%的乙醇8倍量,提取3次,每次1h,浸膏得率为17.33%,天麻素的平均含量为0.35%,RSD=1.9%(n=5);天麻多糖平均含量为13.33%,RSD=4.5%(n=5)。结论天麻提取物制备工艺简便,经济环保,其天麻素和天麻多糖含量控制方法准确可行。更多还原

【Abstract】 OBJECTIVE Study with the extraction conditions of gastrodin and polysaccharide in Gastrodia elate Bl.from Sichuan Pingwu.METHODS Orthogonal experiment design was adopted with Soxhlet extraction.Gastrodin and polysaccharide was examined by TLC 、 UV and colorimetric determination.RESULTS The best extraction solvent was 70% alcohol and 10 times.The time for each extraction was 2 hours and 3 time.The content of gastrodin was 0.35%,RSD =1.9%(n=5) and polysaccharide was 13.33%,RSD = 4.5%(n=5).CONCLUSION The production technique was simple and safe.The menthods of determination with it’s function substances can be estabished.更多还原