【作者】 魏赛金； 涂国全；

【Author】 WEI Sai-jin TU Guo-quan (College of Biological Science and Engineering,Jiangxi agriculture university,Nanchang 330045)

【机构】 江西农业大学生物科学与工程学院；

【摘要】 为了探索珙桐植物内生菌的情况,从珙桐枝条中筛选到1株内生细菌B211,采用形态学、生理生化鉴定结合16SrDNA序列分析及部分特异性基因序列分析,鉴定其为枯草芽孢杆菌。采用生长速率法测定该菌对多种植物病原真菌的作用效果。结果表明,菌株B211对水稻菌核病、水稻纹枯病、棉花黄萎病抑制率分别为75.56%、72.22%、74.44%,抑菌谱较广。由此可见珙桐内生细菌具有作为生物防治菌的应用潜能。更多还原

【Abstract】 Endophytic bacteria strain B221 was isolated from Davidia involucrate Brail.It was identified as Bacillus subtilis according to the morphological,physiological characteristics,16SrDNA sequence analysis and some specific genes analysis.The strain B221 showed the ability of antagonizing some pathogenic fungi through the growth rate method.The effect showed it had obvious antagonisms against Helminthosporium sigmoideum Cav、Rhizoctonia solania and Verticillium dahliae.Rates of restrainability achieved 75.56%、72.22% and 74.44% respectively.The strain B211 had wide disease suppression spectrums,So it could be regard as bacteria protecting biology.更多还原