【作者】 颜伟玉； 曾志将； 吴小波； Yves LE CONTE； Dominique BESLAY；

【Author】 YAN Wei-yu~1,Yves LE CONTE~2,Dominique BESLAY~2,ZENG Zhi-jiang~(1※) (1.Honeybee Research Institute,Jiangxi Agricultural University,Nanchang 330045;2. Laboratoire de Biologie de l’Abeille,INRA,84194 Avignon Cedex 9,France)

【机构】 江西农业大学蜜蜂研究所； Laboratoire de Biologie de l’Abeille,INRA,84194 Avignon Cedex 9,France；

【摘要】 研究分析了中华蜜蜂(Apis cerana cerana)幼虫是否含有与西方蜜蜂(Apismellifera)幼虫相同的幼虫利它素和信息素及其在不同日龄阶段幼虫的分布情况。以中华蜜蜂为实验材料,取2日龄、6日龄(即将封盖)和7日龄(封盖后)的工蜂幼虫及2日龄、7日龄(即将封盖)和8日龄(封盖后)的雄蜂幼虫,经过正己烷浸泡碾碎,取上清液并进行层析过柱分离,应用气相色谱来研究中华蜜蜂幼虫利它素和信息素。本研究首次发现中华蜜蜂幼虫信息素含有甲基棕榈酸酯(MP)、甲基油酸酯(MO)、甲基硬脂酸酯(MS)、甲基亚油酸酯(ML)、甲基亚麻酸酯(MN)、乙基棕榈酸酯(EP)、乙基油酸酯(EO)、乙基硬脂酸酯(ES)、乙基亚油酸酯(EL)和乙基亚麻酸酯(EN)。中华蜜蜂幼虫含有与西方蜜蜂幼虫相同的幼虫利它素和10种脂肪类信息素,但这两种蜜蜂信息素的含量及在不同日龄幼虫分布规律不同。更多还原

【Abstract】 To study and analyze the brood kairmone and pheromone of different stage brood in Apis cerena cerena,and compared it with Apis mellifera.Worker larvae(2 days old,6 days old and 7 days old) and drone larvae(2 days old,7 days old and 8 days old) of Apis cerena cerena were extracted with hexane by crushing,the supernatant were then fractioned by chromatography on a column of silica gel and tested by gas chromatogram.It is first time to identify the components of brood kairmone and pheromone in Apis cerena cerena,methyl palmitate,methyl oleate,methyl stearate,methyl linoleate,methyl linolenate,ethyl palmitate,ethyl oleate,ethyl stearate,ethyl linoleate and ethyl linolenate.The kairmone and 10 aliphatic esters in brood have been found the same between Apis mellifera and Apis cerana cerana,while the content and distribution of the two pheromone are different in different brood stage.更多还原