【作者】 郭时金； 徐倩倩； 周春凤； 张志美； 付石军； 沈志强；

【Author】 GUO Shi-jin1,2 Xu Qian-qian1,2 Zhou chun-feng1,2 Zhang Zhi-mei1,2 Fu Shi-jun1,2 Shen Zhi-qiang1,2(1.Shandong Binzhou Animal Science & Veterinary Medicine Institute,Shandong Binzhou,256600,China;2.Shandong Lvdu Ante Veterinary Drug Industry,Shandong Binzhou,256600,China)

【机构】 山东省滨州畜牧兽医研究院； 山东绿都安特动物药业有限公司；

【摘要】 实时-反转录多聚酶链式反应(rRT-PCR)是分子诊断和研究领域广泛应用的一种方法。灵敏、特异、快速地检测和定量病毒RNA,使其成为人类和动物重要病毒性抗原的诊断必不可少的工具。将该分析方法与自动液相处理平台结合起来进行核酸提取,提高了效率,试样处理量更加标准,减少了交叉感染的可能。通过内标进一步验证试验结果的可信性。以这些特性为基础,已建立并验证了大量有效的针对重要家畜流行性疾病的rRT-PCR系统。在此讨论了该分析方法在病毒诊断、疾病控制和方法的改进。更多还原

【Abstract】 Real-time,reverse transcription polymerase chain reaction(rRT-PCR)has become one of the most widely used methods in the field of molecular diagnostics and research.The potential of this format to provide sensitive,specific and swift detection and quantification of viral RNAs has made it an indispensable tool for state-of-the-art diagnostics of important human and animal viral pathogens.Integration of these assays into automated liquid handling platforms for nucleic acid extraction increases the rate and standardization of sample throughput and decreases the potential for cross-contamination.The reliability of these assays can be further enhanced by using internal controls to validate test results.Based on these advantageous characteristics,numerous robust rRT-PCRs systems have been developed and validated for important epizootic diseases of livestock.The performance of these tests for viral diagnostics and disease control and prospects for improved strategies in the future are discussed.更多还原