【作者】 孙仁爽； 金哲雄； 姜启娟；

【Author】 SUN Ren-shuang~(1,2),JIN Zhe-xiong~2,JIANG Qi-juan~3 (1 Tonghua Normal University,Tonghua 134001,China;2 Harbin University of Commerce,Harbin 150076,China 3 Tonghua Institute for Control of Food and Drug,Tonghua 134001,China)

【机构】 通化师范学院； 哈尔滨商业大学； 通化市食品药品检验所；

【摘要】 目的:建立肾衰宁丸质量标准。方法:采用反相高效液相色谱法,色谱柱为依利特C18（4.6mm×200mm,5μm）;流动相为乙腈-0.05 mol·L^-1磷酸二氢钾（30:70）;检测波长为349 nm;流速为0.8 mL·min^-1。结果:盐酸小檗碱在O.051μg-0.459μg范围王呈线性关系;平均回收率为99.95%,RSD=0.12%。结论:所建立的方法可简便、准确地测定肾衰宁丸中盐酸小檗碱的含量,可用于该制剂的质量控制。更多还原

【Abstract】 Objective:To establish the quality standard of Shenshuaining Pills.Methods:The method for determination of berberine hydrochloride in Shenshuaining Pills by RP-HPLC was established.The column was C₁₈ with Acetonitrile-0.05%Phosphate acid（30:70） as t he mobile phase.The detection wavelength was at 349nm,The detection flow was 0.8 mL·min^-1.Results:The standard curves was linear relationship at t he rang of 0.0624～0.5616μg.The average recovery was 99.95%.RSD was 0.12%. Conclusion:The method is simple,accurate,which is suitable for quality control of the pills.更多还原