【作者】 武夏明； 孙兆荣； 郭丰广；

【Author】 WU Xia-ming,SUN Zhao-rong,GUO Feng-guang(The Affiliated Hospital of Taishan Medical College,Shandong Taian 271000,China)

【机构】 泰山医学院附属医院；

【摘要】 目的:建立测定酮通颗粒中总黄酮醇苷含量的方法。方法:用高效液相色谱法测定酮通颗粒中总黄酮醇苷含量的含量,色谱柱为Diamonsil^TM C₁₈（250mm×4.6 mm,5μm）,以甲醇-0.4%磷酸（55:45）为流动相,流速为1.0mL·min^-1,检测波长为360 nm,柱温为35℃。结果:槲皮素、山柰素、异鼠李素的线性范围分别为5.24～83.84μg·mL^-1（r=0.999 9）、4.56～72.96μg·mL^-1（r=0.9999）、2.48～39.68μg·mL^-1（r=0.999 9）,平均回收率分别为99.84%（RSD=1.26%）、99.61%（RSD=1.06%）、99.39%（RSD=1.08%）。结论:所建立的方法准确、可靠,专属性强,可有效控制酮通颗粒的质量。更多还原

【Abstract】 OBJECTIVE To establish a method for determination of total flavonol glycosides in Tongtong granules by HPLC.METHODS The content of total flavonol glycosides in Tongtong granules were determined by HPLC. Chromatographic conditions were as follows:Diamonsil^TM C₁₈ column（250mm×4.6 mm,5μm） as the stationary phase,methanol-0.4%phosphoric acid（55:45） as the mobile phase,the velocity of flow was 1.0 mL·min^-1 the temperature of coloum was 35℃and the detection wavelength was 360 nm.RESULTS The linear ranges of quercetin,kaempferide and isorhamnetin were 5.24-83.84μg·mL^-1（r =0.9999）、4.56-72.96μg·mL^-1（r =0.9999）、2.48-39.68μg·mL^-1（r =0.9999）, respectively.The average recovery were 99.84%（RSD =1.26%）,99.61%（RSD =1.06%）、99.39%（RSD =1.08%） respectively.CONCLUTION The method was accurate,reliable and strong specificity.,which can be used to contral the quality of Gengmaitong granules.更多还原