【作者】 武夏明； 孙兆荣；

【Author】 WU Xia-ming,SUN Zhao-rong. (The Affiliated Hospital of Taishan Medical College,Taian 271000,China)

【机构】 泰山医学院附属医院；

【摘要】 目的:建立田银通脉颗粒中三七总皂苷中三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁的含量测定方法。方法:采用高效液相色谱法测定田银通脉颗粒中三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁的含量。色谱柱为Diamonsil^TM C₁₈（250 mm×4.6 mm,5μm）;流动相:乙腈（A）-0.05%磷酸水（B）梯度洗脱（0～8 min,20%A:8～40 min 20%A～30%A;40～60 min,30%A～45%A）;流速为1.0mL·min^-1;柱温为室温;检测波长:203 nm。结果:三七皂苷R1在0.212～3.392μg范围内线性关系良好,r=0.999 6,平均回收率为100.21%,RSD=1.71%（n=9）;人参皂苷Rg₁在0.832～13.312μg范围内线性关系良好,r=0.999 5,平均回收率为100.24%,RSD=1.62%（n=9）,人参皂苷Rb₁在0.816～13.056μg范围内线性关系良好,r=0.999 7,平均回收率为99.66%,RSD=1.59%（n=9）。结论:所建立的方法准确、可靠,专属性强,可有效控制田银通脉颗粒的质量。更多还原

【Abstract】 OBJECTIVE:To establish a method for determination of notoginsecosideR₁,ginsenosideRg₁ and Rb₁ in Tianyintongmai granules by HPLC. METHODS:The content of notoginsecosideR₁,ginsenosideRg₁ and Rb₁ in Tianyintongmai granules were determined by HPLC.Chromatographic conditions were as follows:Diamonsil^? C₁₈ column（250 mm×4.6 mm,5μrn） as the stationary phase,acetonitrile（A）- 0.05%phosphoric acid（B）（0—8 min,20%A;8—40 min 20%A³0%A;40—60 min,30%A—45%A） as the gradient mobile phase,the velocity of flow was 1.0 mL·min^-1,the temperature of coloum was room temperature and the detection wavelength was 203 nm.RESULTS:The linear ranges of notoginsecosideR₁,ginsenosideRg₁ and Rb₁ fell within 0.212～3.392μg（r =0.999 6）,0.832-13.312μg（r =0.999 5）,0.816～13.056 ug（r =0.999 7）,respectively. The average recovery were 100.21%（RSD =1.71%,n = 9）,100.24%（RSD = 1.62%,n = 9）,99.66%USD =1.59%,n = 9),respectively.CONCLUTION: The method was accurate,reliable and strong specificity.,which can be used to contral the quality of Tianyintongmai granules.更多还原