【作者】 王林； 朱希强；

【Author】 WANG Lin,ZHU Xi-qiang (Institute of Biopharmaceuticals of Shandong Province,Jinan 250101,China)

【机构】 山东省生物药物研究院；

【摘要】 目的筛选ε-聚赖氨酸高产菌株。方法利用紫外和亚硝基胍复合诱变,在筛选平板中添加赖氨酸结构类似物AEC和ε-聚赖氨酸。结果筛选获得了高产菌株NTG-46,摇瓶中的产量达到1.84g/L,比原始菌株提高了55.9%。结论在白色链霉菌合成ε-聚赖氨酸的过程中,存在赖氨酸的反馈抑制和终产物抑制,赖氨酸结构类似物AEC和ε-聚赖氨酸,有效解除了抑制作用,筛选获得了高产菌株。更多还原

【Abstract】 Purpose To screen high-yield strain ofε-poly-L-lysine.Methods the strain was mutated by sequence of UV and nitrosoguanidin,and AEC andε-poly-L-lysine were added to the screening media.Results Theε-poly-L-lysine yield of selected strain NTG-46 is 1.84g/L increasing by 55.9%compared with ordinary strain. Conclusion The resistant mutant to AEC ande-poly-L-lysine has no feedback inhibition,and the yield ofε-poly-L-lysine was evaluated.更多还原