【作者】 王桂兰； 张晓元； 陈晓燕； 朱希强； 凌沛学；

【Author】 WANG Gui-lan~(1,2),ZHANG Xiao-yuan~2,CHEN Xiao-yan~2,ZHU Xi-qiang~(1,2),LING Pei-xue~(1,2) (1.School of Pharmaceutical Science,Shandong University,Jinan 250012,China;2.Postdoctoral Scientific Research Workstation,Institute of Biopharmaceuticals of Shandong Province,Jinan 250101,China)

【机构】 山东大学药学院； 山东省生物药物研究院博士后科研工作站；

【摘要】 目的通过加入表面活性剂,提高黄原胶发酵产量。方法初步筛选6种常用的表面活性剂,优选两种优势互补的表面活性剂进行加入量、加入时间、加入比例的优化,采用单因素和正交试验得到最佳工艺参数。结果 Tween-20、Triton X-100协同使用效果最好,发酵0h加入0.06%(v/v)Triton X-100,48h加入0.09%(v/v)Tween-20,黄原胶产量提高42.0%。结论表面活性剂能提高黄原胶发酵产量。更多还原

【Abstract】 Purpose To improve the fermentation yield of xanthan gum by adding surfactants.Methods Six surfactants were tested as accelerants,and chose two to optimize the addition dosage,addition time and addition ratio respectively. Monofactorial experiments and orthogonal test were investigated to find the combined effect of the two kinds of surfactants.Results Tween-20 and Triton X-100 were the best,adding 0.06%(v/v)Triton X-100 in the beginning of fermentation,adding 0.09 %(v/v)Tween-20 at 48 h in the fermentation period,the yield of xanthan gum could be improved 42.0%.Conclusion Surfactants can promote xanthan gum production.更多还原