【作者】 周鹏； 陈刚； 王博； 张成； 刘文敏； 魏然； 栾萌； 杨树林； 刘阳； 高春义；

【Author】 ZHOU Peng,CHEN Gang,Wang Bo et al. Key laboratory of medical genetics,Institute of basic medicine,Shandong academy of medical sciences,Jinan,250062,China

【机构】 山东省医学科学院基础医学研究所医学遗传学重点实验室； 山东省济南市第五人民医院；

【摘要】 目的:对山东省原发性高血压患者群体的2号染色体进行扫描,寻找关联区域,定位易感基因。方法:用DNA混合池(DNApooling)方法,在2号染色体上间隔10 cM(厘摩)遗传距离选择了30个微卫星遗传标记,对450例原发性高血压患者和450例正常对照者组成的DNA混合样本分别进行了扫描。采用CLUMP软件进行统计学分析,比较患者组与对照组每个等位基因频率的差异。结果:在D2S2211(2p25.1)位点患者组与对照组的等位基因频率差异有显著性意义,p<0.01。结论:山东省原发性高血压患者群体在2号染色体D2S2211位点存在关联,附近可能存在易感基因,需进一步筛查。更多还原

【Abstract】 Objective A search for susceptibility loci to essential hypertension on chromosome 2 was performed for finding susceptibility loci for essential hypertension.Methods A total of 30 microsatellite markers on chromosome 2 spaced at about 10 cM were selected and two separated DNA pooling samples consisting of 450 essential hypertension cases and 450 normal controls were genotyped respectively.Statistic analysis was performed by Clump software to compare the difference in allele frequency between two pooled samples.Results Significant statistic difference in alleles frequency was found at D2S2211 between cases and controls and the Pvalue was<0.01.Conclusion D2S2211 on chromosome 2 was associated to essential hypertension in Shandong Province and further screen of candidate genes is needed around this locus.更多还原