Transcriptome analysis of grass carp spleen during GCHV infection

【作者】 许宝红； 陈开健； 肖调义； 苏建明； 刘巧林； 钟蕾； 王红权； 戴拥军；

【Author】 Baohon Xu;Kaijian Chen;Tiaoyi Xiao;Jianming Su;Qiaolin Liu;Lei Zhong;Hongquan Wang;Yongjun Dai;

【机构】 湖南农业大学动物科学技术学院；

【摘要】 Background:Grass carp(Ctenopharyngodon idellus)is one of four major freshwater fish in China and,among freshwater species,is the most cultivated for food.Aquacultural development of grass carp has been seriously hindered by grass carp hemorrhage virus(GCHV),which causes severe internal and external hemorrhaging.Here,we performed transcriptome analysis of grass carp during infection to identify important genes in the defense against GCHV.Results:We constructed a Solexa c DNA library from the spleen of fish infected with GCHV.Transcriptome sequencing was performed using the Roche454 platform.High-throughput,paired-end sequencing yielded 27,511,114 reads(644,888 contigs)in the infected group and 27,222,226 reads(892,926 contigs)in the healthy group.Annotation was completed for 95,639 unigenes in the infected group and for 131,904 unigenes in the healthy group.Gene ontology and KEGG analysis classified all unigenes into functional categories and mapped them into specific pathways.We found that 32,621 unigenes were differentially expressed and 9,916 unigenes were enriched in 217 pathways.Of these,the four most highly involved pathways included the complement and coagulation cascades,hematopoietic cell lineage,phagosome and cytokine-cytokine receptor interactions.Among these four pathways,we identified 13 differentially expressed factors closely related to the hemorrhagic symptoms associated with GCHV infection.Conclusion:The transcriptome analysis of grass carp identified several factors associated with GCHV infection.Modulating these factors could help aquacultural efforts as well as inform genetic programs designed to breed disease-resistant strains of grass carp and other cyprinids.更多还原

【Abstract】 Background:Grass carp(Ctenopharyngodon idellus) is one of four major freshwater fish in China and,among freshwater species,is the most cultivated for food.Aquacultural development of grass carp has been seriously hindered by grass carp hemorrhage virus(GCHV),which causes severe internal and external hemorrhaging.Here,we performed transcriptome analysis of grass carp during infection to identify important genes in the defense against GCHV.Results:We constructed a Solexa c DNA library from the spleen of fish infected with GCHV.Transcriptome sequencing was performed using the Roche 454 platform.High-throughput,paired-end sequencing yielded 27,511,114 reads(644,888 contigs) in the infected group and 27,222,226 reads(892,926 contigs) in the healthy group.Annotation was completed for 95,639 unigenes in the infected group and for 131,904 unigenes in the healthy group.Gene ontology and KEGG analysis classified all unigenes into functional categories and mapped them into specific pathways.We found that 32,621 unigenes were differentially expressed and 9,916 unigenes were enriched in 217 pathways.Of these,the four most highly involved pathways included the complement and coagulation cascades,hematopoietic cell lineage,phagosome and cytokine-cytokine receptor interactions.Among these four pathways,we identified 13 differentially expressed factors closely related to the hemorrhagic symptoms associated with GCHV infection.Conclusion:The transcriptome analysis of grass carp identified several factors associated with GCHV infection.Modulating these factors could help aquacultural efforts as well as inform genetic programs designed to breed disease-resistant strains of grass carp and other cyprinids.更多还原