The neuroprotective effects of baicalin on ethanol-induced impairment via anti-oxidative and anti-apoptotic pathway

【作者】 王鹏； 曲丽辉； 曹永刚； 张倩龙； 李淑芝； 朱辉；

【机构】 哈尔滨医科大学大庆校区基础医学院生理教研室； 哈尔滨医科大学大庆校区基础医学院机能实验中心； 哈尔滨医科大学基础医学院生理教研室；

【摘要】 Baicalin is a major bioactive ingredient from the roots of Scutellaria baicalensis Georgi and has various pharmacological actions such as anti-apoptosis and anti-oxidative stress.However,an involvement of baicalin in the ethanol-induced cerebral impairment remains unknown.Therefore,here we explored that the neuroprotective effects of baicalin on ethanol-induced impairment in C57BL/6 mice model with Lieber-De Carli liquid diet including ethanol.By utilizing Morris water maze test,we found that baicalin attenuated the cognitive dysfunction of mice treated with ethanol.To discuss the anti-apoptosis effects of baicalin on ethanol mediated neuronal impairment,histopathological examination such as Nissl staining and Neu N immunohistochemistry,molecular biological analysis such as Western Blot and quantitative PCR were used to evaluate the apoptosis of hippocampal neurons.The analysis of above results showed that baicalin could attenuate apoptosis of hippocampal neurons after ethanol impairment.To explore the anti-oxidative mechanism of baicalin in hippocampal neuronal impairment caused by ethanol,the oxidative stress injury of hippocampal neurons was evaluated by measuring levels of malondialdehyde(MDA),activities of catalase(CAT),Cu/Zn-superoxide dismutase(SOD),Mn-SOD,glutathione(GSH)and glutathione peroxidase(GSH-PX).In addition,Western blot analysis showed that baicalin modulated the protein expressions of mitogen-activated protein kinases(MAPKs),including ERK,JNK and p38.Therefore,these results suggest that baicalin alleviates ethanol-mediated hippocampal neuronal impairment by anti-apoptosis and anti-oxidative,and then improves cognitive function of mice treated byethanol,which is likely to be related to MAPK signal pathway.更多还原

【Abstract】 Baicalin is a major bioactive ingredient from the roots of Scutellaria baicalensis Georgi and has various pharmacological actions such as anti-apoptosis and anti-oxidative stress.However,an involvement of baicalin in the ethanol-induced cerebral impairment remains unknown.Therefore,here we explored that the neuroprotective effects of baicalin on ethanol-induced impairment in C57BL/6 mice model with Lieber-De Carli liquid diet including ethanol.By utilizing Morris water maze test,we found that baicalin attenuated the cognitive dysfunction of mice treated with ethanol.To discuss the anti-apoptosis effects of baicalin on ethanol mediated neuronal impairment,histopathological examination such as Nissl staining and Neu N immunohistochemistry,molecular biological analysis such as Western Blot and quantitative PCR were used to evaluate the apoptosis of hippocampal neurons.The analysis of above results showed that baicalin could attenuate apoptosis of hippocampal neurons after ethanol impairment.To explore the anti-oxidative mechanism of baicalin in hippocampal neuronal impairment caused by ethanol,the oxidative stress injury of hippocampal neurons was evaluated by measuring levels of malondialdehyde(MDA),activities of catalase(CAT),Cu/Zn-superoxide dismutase(SOD),Mn-SOD,glutathione(GSH) and glutathione peroxidase(GSH-PX).In addition,Western blot analysis showed that baicalin modulated the protein expressions of mitogen-activated protein kinases(MAPKs),including ERK,JNK and p38.Therefore,these results suggest that baicalin alleviates ethanol-mediated hippocampal neuronal impairment by anti-apoptosis and anti-oxidative,and then improves cognitive function of mice treated byethanol,which is likely to be related to MAPK signal pathway.更多还原