【作者】 杨雨玲； 李伟； 陈伟洲； 徐军田；

【Author】 YANG Yu-ling 1 , Li Wei 2 , Chen Wei-zhou 1 , Xu Jun-tian 2,3 (1.Marine biology institute, Shantou University,Shantou Guangdong 515063; 2. State key laboratory of marine environmental science, Xiamen University, Xiamen Fujian 361005; 3. School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, 222005)

【机构】 汕头大学海洋生物研究所； 厦门大学近海海洋环境科学国家重点实验室； 淮海工学院海洋学院；

【摘要】 为了探索大型海藻生活史初级阶段对于海洋酸化与紫外辐射的响应,本文选取经济海藻坛紫菜(Porphyra haitanensis)的自由丝状体作为实验材料。实验设置两个CO2浓度,低CO2浓度(390ppmv)和高CO2浓度(1000ppmv);三种辐射处理,PAR处理(仅接受可见光)、PA处理(滤除UV-B)、PAB处理(全波长辐射)。研究结果表明,高CO2显著提高了坛紫菜丝状体的生长速率,但高CO2处理下坛紫菜自由丝状体中藻红蛋白、藻蓝蛋白、叶绿素a,类胡萝卜素及紫外吸收物质UVACs分别降低了7.3%、9.2%、19.8%,16.45%和18.7%。高CO2处理的坛紫菜自由丝状体最大光化学效率Fv/Fm,光能利用效率(α)及最大相对电子传递速率(rETRmax)都显著高于低CO2处理。太阳模拟器下处理的坛紫菜自由丝状体,PAR与PA处理下的抑制率,低CO2与高CO2处理间无显著差异,然而在PAB处理下,高CO2处理的抑制率要高于低CO2处理,这可能与其体内紫外吸收物质含量下降有关。PAR处理下的抑制率,无论是在低CO2还是在高CO2处理下,都显著低于PA及PAB处理,而PA与PAB之间无显著差异。在未来海洋持续酸化的背景下,UVR辐射的增加将会影响到坛紫菜自由丝状体的光合生理及产量。更多还原

【Abstract】 To study the effects of ocean acidification and UV radiation on early life cycle stage of macroalgae, we selected the economic algae conchocelis of Porphyra haitanensis as our material. Two CO 2 levels (low CO 2 , 390 ppmv and high CO 2 , 1000 ppmv) and three irradiances( PAR (400-700nm), PA (315-400nm) and PAB (280-400nm)) were set in the study. Our results showed that high CO 2 significantly enhanced the relative growth rate of conchocelis of Porphyra haitanensis, but the contents of phycoerythrin, phycocyanin, chlorophyll a, carotenoid and UVACs were significantly reduced by 7.3%, 9.3%, 19.8%, 16.45% and 18.7% under high CO 2 condition, respectively. Maximum photochemical efficiency (F v /F m ), light using efficiency(α) and maximum relative electron transport rate(rETR max )were all significantly increased under high CO 2 condition. When exposed to different light treatments, inhibition induced by PAR and PA were no significant differences between the thalli grown at high and low CO 2 . However, significant inhibition under PAB was found in the thalli grown at high CO 2 ,which was closed related to the low concentration of UVACs in the thalli of conchocelis of Porphyra haitanensis. Inhibition induced by PAR were all lower than PA and PAB in both high and low CO 2 treatments, however, no difference were found between PA and PAB treatments. In future ocean, increased UVR would show great effects on photosynthesis and production of Porphyra haitanensis, especially under the background of ocean acidification.更多还原