【作者】 曹海鹏； 安健； 陈百尧； 伏光辉； 吕利群；

【Author】 CAO Hai-peng 1* , AN Jian 2 , CHEN Bai-yao 2 , FU Guan-hui 2 , LV Li-qun 1* (1.National Pathogen Collection Centre for Aquatic Animals, Shanghai Ocean University, Shanghai, 201306, China; 2. Luxury Seafood Culture and Enhancement of Lianyungang, Marine and Fisheries Research Institute of Lianyungang, Lianyungang, Jiangsu, 222044, China)

【机构】 上海海洋大学国家水生动物病原库； 连云港市海洋与水产科学研究所连云港海珍品增养殖试验场；

【摘要】 评价对鲟源嗜水气单胞菌具有优良拮抗效果的解淀粉芽孢杆菌G1的安全性,以期推动其在水产养殖中的应用进程。通过特异PCR法检测了菌株G1的毒力基因,并采用纸片法分析了其对水产药物的耐药性,观察了其对小白鼠和草鱼的毒力。实验结果表明,菌株G1不含有溶血素基因、肠毒素T基因、肠毒素FM基因,对诺氟沙星、恩诺沙星、新霉素、复方新诺明、甲氧嘧啶、链霉素、氧氟沙星、庆大霉素、复合磺胺等9种水产常用药物以及呋喃唑酮、环丙沙星、氯霉素、红霉素等4种水产禁用药物高度敏感,对四环素中度敏感。此外,菌株G1在105~109cfu/g体重注射剂量下对小白鼠和草鱼无致病性,对小白鼠和草鱼的LD50大于109cfu/g体重,为无毒菌株。更多还原

【Abstract】 The present study was to evaluate the safety of B. amyloliquefaciens strain G1 against sturgeon-pathogenic Aeromonas hydrophila, and promote its application in aquaculture. The virulence genes of B. amyloliquefaciens strain G1 were detected using specific PCR, its susceptibility against fishery and veterinary drugs was assayed using the disc diffusion method, and its pathogenicity to mice and grass carps was tested. The experimental results showed that the hemolysin (hlyA) gene and two enterotoxin (becT and entFM) genes were not present in the G1 strain. Strain G1 exhibited highly susceptible to 9 common fishery drugs (norfloxacin, enrofloxacin, neomycin, trimethoprim-sulfamethoxazole, sulfamethoxydiazine, streptomycin, ofloxacin, gentamicin, syntactic sulfonamide) and 4 anti-doping fishery drugs (furazolidone, ciprofloxacin, chloramphenicol, erythromycin), intermediately susceptible to tetracycline. In addition, the G1 strain was confirmed to be no pathogenic to mice and grass carps at the cell densities of 10 5 ~10 9 cfu/g body weight, and its lethal doses of 50% was estimated as above 10 9 cfu/g body weight. Thus, the G1 strain was safe in use.更多还原