【作者】 胡毅； 黄云； 文华； 钟蕾； 肖调义；

【Author】 Huang Yun 1 ,Hu Yi 1* , Wen Hua 2 , Zhonglei 1 , Xiao Tiao-yi 1 (1. College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;2.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China)

【机构】 湖南农业大学动物科学技术学院； 中国水产科学研究院长江水产研究所淡水生态与健康养殖重点开放实验室；

【摘要】 以初始体质量7.27±0.40g的青鱼为研究对象,研究VE对青鱼幼鱼生长、免疫及抗氨氮胁迫的影响。结果表明:以特定生长率为指标,折线模型表明青鱼有效维生素E需要量为65.0mg/kg。VE对鳃丝Na+/K+-ATP酶活性(NKA)和血清皮质醇(COR)无显著影响,但随着饲料VE含量的升高,过氧化氢酶(CAT)和总超氧化物歧化酶活性(T-SOD)呈上升趋势,丙二醛含量(MDA)呈下降趋势。氨氮胁迫使肝脏VE含量均显著降低(P<0.05),但VE添加组血清VE水平有所升高。胁迫后,对照组血清T-SOD、鳃丝NKA活性显著降低,皮质醇含量显著增加(P<0.05),VE添加组T-SOD、NKA活性和皮质醇含量胁迫前后无显著变化。各处理组MDA含量胁迫后虽均显著升高,但400mg/kgVE添加组胁迫后MDA含量显著低于对照组(P<0.05)。以上结果说明,青鱼幼鱼获最大生长有效维生素E需求量为65.0mg/kg,且添加较高VE能有效提高青鱼机体免疫力,缓解氨氮胁迫对青鱼机体的负面影响。更多还原

【Abstract】 Studies were conducted to investigate the effects of dietary vitamin E (VE) on growth, immunity and anti ammonia nitrite stress ability in juvenile black carp, Mylopharyngodon piceus (Initial average weight 7.27±0.40 g). Results indicated that the effective VE content for maximum special growth rate (SGR) is about 65.0 mg/kg. No significant differences in gill Na + /K + -ATPase (NKA) activity were observed among dietary treatments, but with the vitamin E content in diets increased, total superoxide dismutase (T-SOD) and catalase (CAT) activity were increased and malondialdehyde (MDA) content decreased. Each group of muscle vitamin E concentration and CAT activity were not affected by ammonia-nitrite stressed, but liver vitamin E concentration were significant decreased and serum VE level were increased in VE diets. After stressed, T-SOD and gill NKA activity in control group were significant decreased and cortisol content was significant increased (P<0.05). But two vitamin E supplemental group of T-SOD, NKA activity and cortisol were no significant changed. MDA content in each group were significant increased after stressed, however, the 400 mg/kg vitamin E group MDA content was significant lower than control group (P<0.05). All the results indicated that the effective VE requirement of juvenile black carp for optimal growth might be 65.0 mg/kg diet, and higher vitamin E supplement in diet could improve non-immune response and anti ammonia-nitirite stress ability of black carp.更多还原