【作者】 杨方园； 关瑞章； 赖晓健；

【Author】 YANG Fang-yuan,GUAN Rui-zhang 8 ,LAI Xiao-jian (1. Fisheries Colleges,Jimei University,Xiamen 361021,china; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University),Fujian Province,Xiamen 361021,china; 3. Engineering Research Center of the Modern Induatrial Technology for Eel,Ministry of Education,Xiamen 36102,china)

【机构】 集美大学水产学院； 福建省高校水产科学与食品安全重点实验室； 鳗鲡现代产业技术教育部工程研究中心；

【摘要】 从发病花鳗鲡(Anguilla marmorata)肝脏分离得到优势菌株RB6,经人工腹腔注射感染试验证实该菌对健康鳗鲡有致病作用,其半数致死量LD50为4.4×106CFU/ml。通过菌体及菌落形态特征,结合Biolog自动微生物鉴定系统鉴定为弗氏柠檬酸杆菌(Citrobacter freundii)。同时对其16SrRNA序列扩增并测序,在NCBI上序列比对后与GenBank数据库中柠檬酸杆菌属细菌的16SrRNA基因序列同源性达到99%,通过以上实验确定RB6为弗氏柠檬酸杆菌(Citrobacter freundii)。药物敏感性试验表明,该菌株对左氧氟沙星、氟哌酸、庆大霉素、阿奇霉素、强力霉素、卡那霉素等15种药物高度敏感;对头孢他啶、阿莫西林和多粘菌素B中度敏感;对氨苄西林、青霉素、复方新诺明、头孢呋辛、头孢克罗、呋喃唑酮等15种药物耐药。更多还原

【Abstract】 A dominant bacterial strain named RB6 was isolated from the liver of the diseased Anguilla marmorata. The strain was proved to be pathogen by intraperitoneal injection with bacteria suspension, and the LD 50 was 4.4×10 6 CFU/ml.By combinative the morphogenetic and Biolog identification system ,the strain was similar to Citrobacter freundii. Analysis of 16S rRNA gene showed that the strain shared more than 99% identity with C.freundii. Based on morphological, biochemical and phylogenetic analysis, the strain was identified as Citrobacter freundii. The stain had high sensitivity to 15 agents including levofloxacin, intermediately sensitive to 3 agents including ceftazidime, and resistant to 15 agents including penicillin during antibiotic sensitivity to 33 antimicrobial agents.更多还原